Evaluation of Cellular Processes by In Vitro Assays

Evaluation of Cellular Processes by In Vitro Assays

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a ...
[view complete introduction]

US $
15

*(Excluding Mailing and Handling)



Reactive Oxygen Species (ROS)

Pp. 58-67 (10)

Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq

Abstract

The reactive oxygen species produced endogenously are essential to life, being involved in several biological functions. However, when produced at higher levels, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage. The study in the field of ROS associated biological functions and/or deleterious effects requires new sensitive and specific tools in order to enable a deeper insight on its action mechanisms. Here, we discuss several methods related to the detection of reactive oxygen species.

Keywords:

Catalase, Fluorescence, Fluorescent assays, Free radicals, Hydrogen peroxide, Mitochondria, Superoxide dismutase.

Affiliation:

Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.