Evaluation of Cellular Processes by In Vitro Assays

Evaluation of Cellular Processes by In Vitro Assays

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a ...
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Cell Cytotoxicity, Viability and Proliferation

Pp. 44-57 (14)

Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq

Abstract

Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. Cell viability, cell proliferation and many important live-cell functions can be stimulated or monitored with various chemical and biological reagents. The present chapter is aimed to discuss the various available method

Keywords:

Biolumniscence, Bromodeoxyuridine and Clonogenic assay, Carboxyfluorescein diacetatesuccinimidyl ester, Hemocytometer, LDH assay, MTT assay, Trypan blue.

Affiliation:

Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.