Pp. 29-43 (15)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
Apoptosis is a critical process having widespread biological significance;
regulating the differentiation, proliferation, maintenance and sculpturing organs and
tissues, functioning of immune system and the elimination of defective harmful cells.
The word apoptosis is derived from Greek word meaning “απόπτωσις” meaning
“falling off of petals from flowers”. It is actually programmed cell death essential for
normal metabolism. In this chapter, we will provide general overview of various
technical approaches for detecting apoptotic cells. The features, advantages and
disadvantages of different methods are also discussed.
Annexin, Caspases, DNA polymerase, Hoechst stain, Homeostasis,
Luminometry, Membrane-blebbing, Necrosis, Phosphatidyl-serine, Propidium
iodide, TUNEL assay.
Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.