Methods of Transfection
Pp. 20-28 (9)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
In order to evaluate the regulation, expression or activity of a gene, it is
necessary to transfer the gene or its manipulated form into the in vitro systems. Since
the mammalian cells do not uptake the foreign DNA efficiently, the availability of
effective methods for introducing genes into the cells is essential. The transfection
methodology has developed rapidly and diversely. Each year many new products and
technologies are launched with improved efficiency and less cytotoxicity. In the
following chapter, we will discuss the advantages and disadvantages of different
methods of transfection.
Cationic lipid, Diethylaminoethyl-dextran, Electroporation,
Endocytosis, Genome, Gene transfer, Lipofectamine, Liposome, Micromanipulator,
Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.