Evaluation of Cellular Processes by In Vitro Assays

Evaluation of Cellular Processes by In Vitro Assays

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a ...
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Methods of Transfection

Pp. 20-28 (9)

Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq

Abstract

In order to evaluate the regulation, expression or activity of a gene, it is necessary to transfer the gene or its manipulated form into the in vitro systems. Since the mammalian cells do not uptake the foreign DNA efficiently, the availability of effective methods for introducing genes into the cells is essential. The transfection methodology has developed rapidly and diversely. Each year many new products and technologies are launched with improved efficiency and less cytotoxicity. In the following chapter, we will discuss the advantages and disadvantages of different methods of transfection.

Keywords:

Cationic lipid, Diethylaminoethyl-dextran, Electroporation, Endocytosis, Genome, Gene transfer, Lipofectamine, Liposome, Micromanipulator, Retrovirus, Transgene.

Affiliation:

Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.