Evaluation of Cellular Processes by In Vitro Assays

Evaluation of Cellular Processes by In Vitro Assays

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a ...
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Basics of In Vitro Cell Culture

Pp. 1-19 (19)

Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq

Abstract

In order to get the intricate biology of living organisms, the researchers started using the basic unit of life i.e. “Cell” for elucidating the intricate mechanisms. This led to the basis of cell culture studies, where cells grown in controlled artificial conditions simulate the conditions prevailing in natural ones, therefore, presumed to act as those in in vivo conditions. The introduction of cell culture techniques has helped a lot in understanding the physiological processes like cell signalling, neurobiology, cell proliferation, pathogenesis of diseases, apoptosis and even more. In the following chapter, we will discuss the basics of cell culture including the equipments, chemicals and different types of materials required for cell culture. The techniques used for maintenance, preservation and authentication of cell lines are also included.

Keywords:

Antibiotics, Aseptic, Cell Culture, Cell Lines, Cryopreservation, Dimethyl-sulphoxide, Dulbecco’s Minimal Essential Media, Fetal Bovine Serum, Fibroblast, Glutamine, Hood, Incubator, Media, Phase Contrast Microscope, Trypsin.

Affiliation:

Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.