Objective: This research aimed to make comparisons of sensitivity and specificity
between quantitative real time polymerase chain reaction (Q-PCR) and reverse transcription PCR
(RT-PCR) in detecting the ribonucleic acid (RNA) expression levels of hepatitis C virus (HCV).
Methods: 121 patients suffering from hepatitis C and 98 healthy participants with normal liver
functions were identified. The venous blood collections were carried out, were subjected to detect
the expression levels of HCV RNA via Q-PCR and RT-PCR. And then, the data obtained from
these above two detection methods were compared, including the sensitivity and specificity.
Results: In terms of Q-PCR, the positive rate of HCV RNA was 72.16%, which was significantly
higher when compared with 55.26% of RT-PCR. After statistical analysis, the difference between
them was statistically significant (P<0.05). Among the healthy participants, 4 cases were false
positive by means of RT-PCR, there was the possibility of missed diagnosis when the samples
were evaluated by Q-PCR.
Conclusion: The Q-PCR detection technology performed well in testing HCV, with pretty high
sensitivity and specificity. Nevertheless, the false negative results obtained from Q-PCR could not
be avoided. In clinical practice, these above two detection methods should be referred to, in order
to avoid missed diagnosis.