Background: Copaifera multijuga are widely used as medicinal plants in Brazil. Of the
various ethnopharmacological indications of copaiba oleoresins, the antimicrobial activity had been
Objective: This study aimed to evaluate the oleoresin and the hydroalcoholic extract of leaves from
Copaifera multijuga against oral pathogens in the sessile and in the planktonic modes.
Method: Standard strains from the American Type Culture Collection and clinical isolates which
cause both cariogenic and endodontic infections were used.
Results: The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration
(MBC) assays showed that the oleoresin was effective against all the bacterial strains. Assessment of
the antibiofilm activity of hydroalcoholic extract of leaves from C. multijuga against the evaluated
microaerophilic bacteria in the sessile mode gave IC50 values of 318.0 and 695.6 μg/mL against S.
mitis (ATCC 49456) and A. actinomycetemcomintans (ATCC 43717), respectively. As for the assayed
anaerobic bacteria, the hydroalcoholic extract of leaves gave IC50 of 4554.0, 2218.0, and
600.1 μg/mL against F. nucleatum (Clinical isolate), P. gingivalis (ATCC 33277), and P. micros
(Clinical isolate), respectively, whereas the oleoresin afforded IC50 of 357.1 μg/mL against P.
gingivalis (ATCC 33277).
Conclusion: The oleoresin and hydroalcoholic extract of leaves displayed satisfactory activity
against oral pathogens in both sessile and planktonic modes. The oleoresin and hydroalcoholic extracts
of leaves from C. multijuga are potential candidates for the development of new products for
dental and oral care.
Keywords: Copaifera multijuga, dental caries, endodontic infections, biofilm, hydroalcoholic extract, oleoresin
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