Background: Diabetes mellitus is the most common disease in the world. One of the approaches
therapy used to treat diabetes is inhibition of α -glucosidase activity by natural inhibitors
from the plant.
Objective: Our work comes to highlight, in vitro, the effect of phenolic extracts of some Algerian Saharan
Atlas medicinal plants on the activity of α-glucosidase.
Methods: The ethyl acetate extracts were prepared from hydro-methanolic (80:20) extracts of studied
plants. The Folin-Ciocalteu method was used to determine the total phenolic content of the extracts.
The α-glucosidase activity was determined by a microplate spectrophotometer at 405 nm using paranitrophenol
α-D-glucopyranose as substrate.
Results: Among the plants tested Arthrophytum scoparium, Helianthemum lippii, Equisetum arvense
and Cistus villosus exhibited a strong α-glucosidase inhibitory activity. According to IC50 values, Arthrophytum
scoparium exerted the highest inhibitory activity against α-glucosidase (IC50= 5.02 µM)
while a weak inhibitory activity was observed with Equisetum arvense (IC50 =21.88 µM). The kinetic
study revealed a noncompetitive type inhibition for Arthrophytum scoparium (KI = 4.16 µM) and Helianthemum
lippii (KI = 1.46 µM) extracts whereas a full mixed type inhibition (KI = 18.17 µM; KIS =
3.98 µM) and competitive type inhibition (KI = 25 µM) were observed for Cistus villosus and Equisetum
Conclusion: The results suggest that these plants represent a potential source to develop new antidiabetic
drugs for controlling postprandial blood sugar.