Background: Cadmium (Cd2+) and lead (Pb2+) have acute and chronic effects on humans
and other living organisms. In the present work, new, green and accurate dispersive micro solid-phase
extraction (DμSPE) method for the separation and preconcentration of trace amounts of cadmium
(Cd2+) and lead (Pb2+) ions in various food, water and tobacco samples collected from Saudi Arabia
prior to its Flame Atomic Absorption Spectrometric (FAAS) determinations was developed.
Methods: The proposed method was based on a combination of oxidized multiwalled carbon
nanotubes (O-MWCNTs) with a new chelating agent 5-benzyl-4-[4-methoxybenzylideneamino)-4H-
1,2,4-triazole-3-thiol (BMBATT) to enrich and separate trace levels of Cd2+ and Pb2+. The effect of
separation parameters was investigated. The validation of the proposed preconcentration procedure
was performed using certified reference materials.
Results: Analyte recovery values ranged from 95-102%, indicating that the method is highly accurate.
Furthermore, precision was demonstrated by the relative standard deviation (RSD < 3.0%). The limits
of detection were 0.08 and 0.1 μg L−1 for Cd2+ and Pb2+ ions, respectively. The preconcentration factor
Conclusion: The proposed method was used for the estimation of Cd2+ and Pb2+ ion content in various
real samples, and satisfactory results were obtained. The proposed method has high adsorption
capacity, rapid adsorption equilibrium, extremely low LODs, high preconcentration factors and
shortens the time of sample preparation in comparison to classical SPE.