Background: Pullulan is a neutral exopolysaccharide produced aerobically by a yeast-like fungus Aureobasidium
pullulans. The distinct linkage of α (1 → 4) & α (1→ 6) in pullulan impart its nonimmunogenic,
non-mutagenic and non-cancerous traits. These characteristics are being exploited in
food, health care, pharmacy, lithography, cosmetics. During the last four decades, pullulan has had a
breakthrough in diverse fields including gene delivery, wound healing, nano-technology, diagnostics
and also in human reproductive health.
Objective: The present review focused on food grade pullulan, its derivatives, and their applications.
Methods: Deviations in biosynthesis and downstream processing of food grade pullulan have been reviewed
Results: Pullulan can be used for a variety of applications. The food applications of pullulan powder and
film have been thoroughly validated. Pullulan film, being carbon neutral, sustainable, biodegradable and
palatable, provides a superior substitute to conventional petrochemical polythene films. Pullulan films
could be modified to add a variety of special features. These formulations have clearly demonstrated the
significance of pullulan, and its potential for more applications.