Background: Fascioliasis is a neglected tropical disease that affects poor people from poor
and developing countries. In the world, it has been estimated that at least 2.6 million people are affected
with this disease. The International agency for Research on Cancer, states that O. viverrini and C. sinensis,
also liver flukes, are considered as definitive causes of cholangiocarcinoma. However, fascioliasis
caused by F. hepatica has not been associated with cancer to date. There are not any known causative
associations between this parasite and liver cancer (cholangiocarcinoma).
Methods: Chine Hamster Ovary (CHO) cells were treated with F. haepatica extracts and cell proliferation
was assessed by using the indirect method for estimating cell number based on the mitochondrial
activity with MTS cell proliferation reagent. We observed unexpected death of these cells when treated
with F. haepatica extracts.
Results and Conclusion: We now hypothesize that this parasite could be used as a medically-important
trematode pathogen in cancer therapy.