Background: Algae gain is of much importance in food and pharmaceutical industries due
to the presence of rich bioactive compounds.
Methods: A Microwave Assisted Extraction (MAE) was engaged to enhance recovery of bioactive
compounds from Dunaliella bardawil. The effective experimental condition was identified by optimization
using Response Surface Methodology (RSM) coupled with Central Composite Rotatable Design
(CCRD) along with Adaptive Neuro-Fuzzy Inference System (ANFIS). In this connection, four
independent parameters, namely, n-hexane concentration (X1:60-85%), microwave power (X2:15-
25%), radiation-induced temperature (X3:40-60°C) and radiation exposure time (X4:7.5-12.5min) were
Results: The optimum conditions resulted in 72.34-75.86% of biomass (y1) and 46.27-47.17 mg of β-
carotene equivalents/g to biomass (y2). In addition, the corresponding antioxidant activities showed
85.65-85.90% in DPPHsc (y3), 81.30-81.31% in ABTSsc (y4) and 59.65-62.45 µgmol (Fe (II))/g in
FRAP (y5). Further, the bioactive compounds, β-carotene, and lutein were identified through HPLC
and the biomass exhibited significant anticancer activity against oral epithelial cancer cells.
Conclusion: The observed results clearly highlight that the study has great scope and value for the
continuation of further exploitation and application.