Introduction: When environment polluted by complex petrochemical contaminants then
prospective for biological treatment is needed.
Methods: Biosurfactants exhibit biodegradability and anti-microbial properties hence efficacy of production
and kinetics of in situ rhamnolipid production by Pseudomonas aeruginosa and lipopeptide
production by Bacillus subtilis was carried out in a batch process. The need of the specific growth rate
on carbon, nitrogen and phosphorous substrates was expected to monitor using Logistic, Monod, Moser
and Contois type kinetic equation models. Impact of several bioprocess factors and optimization of control
factors were studied.
Results and Discussion: The simulation results show, Contois type model was better model than monod
equation. Contois growth model says that bio mass rate depends upon the concentrations of substrate
and biomass being inhibited at high concentrations of the biomass predicts, bio surfactant formation
lowered with the high biomass concentration.
Conclusion: The Luedeking Piret constants were calculated for rahamnolipid and lipopeptide production
bearing in mind the cell survival maintenance from carbon, nitrogenand phosphorous substrates
consumption. Assessed factors of this model specify that the formation of Rhamnolipid and lipopeptide
was typically growth-associated.