Background and Objectives: A rapid, simple and environmental friendly supramolecular
solvent (SUPRAS) based liquid-liquid microextraction method for preconcentration of ciprofloxacin
(CIPRO), danofloxacin (DANO) and enrofloxacin (ENRO) from wastewater was developed.
Methods: This microextraction technique was coupled with high-performance liquid chromatography
equipped with a diode array detector (HPLC-PDA) for detection and separation of the antibiotics. The
SUPRAS composed of decanoic acid and tricaprylymethylammonium chloride. Optimum conditions for
the extraction and preconcentration of all the antibiotics were obtained using surface response methodology
(RSM) based on Box-Behnken design.
Results: Under optimum conditions, the limits of detection (LOD) and limit of quantification (LOQ)
ranged from 0.06-0.14 µg L−1 and 0.22-0.47 μg L−1, respectively with the preconcentration factors ranging
from 153-241. The linear dynamic ranges were between LOQ and 850 µg L−1 with correlation coefficients
ranging from 0.9928 to 0.9999. The intra-day (n = 15) and inter-day (n = 5) precisions (expressed
in terms of %RSD) for 50 µg L−1 of CIPRO, DANO and ENRO were in the range of 3.3–4%
and 4.1–5.8%, respectively.
Conclusion: Lastly, the developed method was used for the extraction, preconcentration and quantification
of selected CIPRO, DANO and ENRO in influent and effluent wastewater samples.