Biofilm Destruction on Endotracheal Tube-associated Pneumonia by photodynamic Inactivation

(E-pub Abstract Ahead of Print)

Author(s): Kate Cristina Blanco*, Amanda C. Zangirolami, Natalia M. Inada, Vanderlei S. Bagnato.

Journal Name: Infectious Disorders - Drug Targets


Hospital infections is a public health problem that can occur with the use of catheters and endotracheal tubes (ETT). Pathogenic microorganisms may adhere to surfaces of these materials forming biofilm. Microbial biofilms presents an extracellular polymer matrix that promotes resistance of microorganisms to factors such as pH, temperature and drugs. The ETT is used to assist a patient with compromised breathing function. ETT biofilm may cause ventilator-associated pneumonia. Pseudomonas aeruginosa and Staphylococcus aureus pneumonia is associated with a 21-fold increase in risk for mechanical ventilation. According to the World Health Organization 1.6 million people die every year from pulmonary infections. These human health problem have have high costs. The conventional treatment is be made by antibiotics, which has serious adverse effects in immunocompromised patients. Bronchoaspiration is the preventive treatment for these diseases in bedridden patients using ETT. Photodynamic therapy (PDT) is an alternative for microbial inactivation noninvasive without stimule of microbial resistance. PDT combines light and a photosensitive molecule for produce reactive oxygen species leading to bacterial death. The objective of this study was to determine the efficacy of a PDT protocol in bacterial inactivation of biofilm ETT. The photosensitizer (PS) used was curcumin and the light source LED at 450nm. Statistical experimental design was used for optimization of antimicrobial PDT. The highest microbial inactivation was found with 70% biofilm reduction in conditions 1 mg/mL curcumin, 2 h of PS incubation and 30 J/cm2.

Keywords: Endotracheal Tube, biofilm; Staphylococcus aureus, Photodynamic therapy

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(E-pub Abstract Ahead of Print)
DOI: 10.2174/1871526518666180523085754
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