Background: Structural similarity in Chalcone and Pyrazoline brought our intention for
the analysis of such compounds. This study involved the synthesis of chalcones and their pyrazoline
derivatives and their screening as cholinesterase inhibitors. The newly synthesized compounds were
also investigated for their antioxidant potential.
Methods: Chalcones were synthesized by well-established methods of synthesis and their structural
elucidation was carried out by H-NMR, 13C-NMR, Mass spectrometry and FTIR. For the determination
of inhibition potency of synthesized compounds, spectrophotometric method was applied
whereas, DPPH free radical scavenging method was used to check the antioxidant ability.
Results: Chalcones and their pyrazoline derivatives were synthesized and characterised by 1HNMR,
13C-NMR, Mass spectrometry and FTIR. The compounds were screened for their anti-
Alzheimer activity, which exhibited that compounds 1g, 1c and 1h, 1g showed strong inhibitory
potency against acetylcholinesterase and butyrylcholinesterase, respectively. DPPH radical scavenging
method was applied to check anti-oxidant potential of synthesized compounds and results
explored that among all the synthesized compounds only compounds 1c and 1b showed strong
Conclusion: Chalcone and their pyrazoline derivatives were synthesized and screened for their
anti-Alzheimer and antioxidant potential. The experimental results of anti-Alzheimer activity were
compared with molecular docking studies, which showed that compounds 1g, 1c and 1h, 1g were
active against AChE and BChE, respectively. Among the synthesized compounds 1c and 1b were
found to be most potent antioxidants. These results suggest that compound 1b, 1c, 1g and 1h may
further be explored for further developments.