Background: Determination of the accurate mass of glucose generated from high-solids
biomass saccharification is vital but problematic due to the uncertainty of liquid volume and slurry density.
Methods: Herein, a new multiple-dilution-assays method was established to deduce the accurate glucose
mass from the hydrolyzing biomass slurry.
Results: This method was applicable for slurries of pretreated corn stover with a solids consistency up
to 30 wt%, showing a high accuracy and good reproducibility. Dryness did not interfere with the accuracy.
Ethanol at a high level, e.g. 10%, caused only a small negative error (<2%). This method can be
used in either single- or fed-batch high-solids biomass saccharification, allowing to quantify the maldistribution
of glucose in the slurry.
Conclusion: The significant advantage of the present method was that only one single variable, glucose
concentration, was to be determined, rendering it unnecessary to wash the insoluble or to measure the
changing liquid density.
Keywords: Multiple dilution assays (MDA), Glucose yield, High-solids loadings, Enzymatic hydrolysis (saccharification), Biomass slurry, Glucose distribution, Fed-batch process
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