Introduction: Lactate minimum test (LMT) identifies a sustainable exercise intensity, in
which an equilibrium is observed between production and clearance of blood lactate and the hormone
influence during this physiological moment.
Objective: The present study aimed to identify the levels of LM and hormones after caffeine consumption
and exercise stress test (ST) in diabetic rats.
Material and Methods: This study was composed of 24 animals, of 60 days, allocated into four
groups: Control, Diabetic, Caffeine, and Diabetes+Caffeine. The Diabetes model was induced by intraperitoneal
administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine were administrated
30 minutes before the exercise stress test (ST) protocol. During the ST animals underwent a
stress test (ST), in which they performed forced swimming (until exhaustion) tie to loads of 13%
body’s weight (bw). The incremental phase of LM began with an initial load of 4% body’s weight
(bw) and increased 0.5% every 5 min. Lactate concentration was measured 5, 7 and 9 min (mmol/L)
after ST. The incremental progressive test (IPT) involved swimming with loads of 4.0, 4.5, 5.0, 5.5,
6.0, and 7.0% of bw, for 5min with each. Blood samples were collected by a caudal puncture to
subsequent lactate and hormone assay.
Results: Performance time and lactate concentration of hyperlactatemia test, as well as lactate minimum
(LM) and lactate (LAC) concentration after the progressive test presented a significant difference
when comparing the levels of the control group with caffeine and diabetic group (p<0.05).
Conclusion: It is suggested that caffeine improves lactate clearance and hormonal steady state condition
of diabetic animals after hyperlactacidemia and physical exercise maintenance.