Caffeine Improves Hormonal and Lactate Homeostasis in Diabetic Rats

(E-pub Ahead of Print)

Author(s): Luiz Augusto Silva*, Jessica Wouk, Vinicius Weber, Pablo Almeida, Julio Cesar Martins, Carlos Malfatti, Raul Osiecki.

Journal Name: Current Nutrition & Food Science

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Abstract:

Introduction: Lactate minimum test (LMT) identifies a sustainable exercise intensity, in which an equilibrium is observed between production and clearance of blood lactate and the hormone influence during this physiological moment. Objective: The present study aimed to identify the levels of LM and hormones after caffeine consumption and exercise stress test (ST) in diabetic rats. Material and Methods: This study was composed of 24 animals, of 60 days, allocated into four groups: Control, Diabetic, Caffeine, and Diabetes+Caffeine. The Diabetes model was induced by intraperitoneal administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine were administrated 30 minutes before the exercise stress test (ST) protocol. During the ST animals underwent a stress test (ST), in which they performed forced swimming (until exhaustion) tie to loads of 13% body’s weight (bw). The incremental phase of LM began with an initial load of 4% body’s weight (bw) and increased 0.5% every 5 min. Lactate concentration was measured 5, 7 and 9 min (mmol/L) after ST. The incremental progressive test (IPT) involved swimming with loads of 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0% of bw, for 5min with each. Blood samples were collected by a caudal puncture to subsequent lactate and hormone assay. Results: Performance time and lactate concentration of hyperlactatemia test, as well as lactate minimum (LM) and lactate (LAC) concentration after the progressive test presented a significant difference when comparing the levels of the control group with caffeine and diabetic group (p<0.05). Conclusion: It is suggested that caffeine improves lactate clearance and hormonal steady state condition of diabetic animals after hyperlactacidemia and physical exercise maintenance.

Keywords: Metabolism, Lactate, Conditioning

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1573401314666180515143951
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