Caffeine Improves Hormonal and Lactate Homeostasis in Diabetic Rats

Author(s): Luiz A. da Silva*, Jéssica Wouk, Vinícius M.R. Weber, Pablo de Almeida, Julio C.L. Martins, Carlos R.M. Malfatti, Raul Osiecki.

Journal Name: Current Nutrition & Food Science

Volume 15 , Issue 6 , 2019

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Abstract:

Introduction: Lactate Minimum Test (LMT) identifies a sustainable exercise intensity, in which an equilibrium is observed between production and clearance of blood lactate and the hormone influence during this physiological moment.

Objective: The present study aimed to identify the levels of LM and hormones after caffeine consumption and exercise Stress Test (ST) in diabetic rats.

Methods: This study was composed of 24 animals, of 60 days, allocated into four groups: Control, Diabetic, Caffeine, and Diabetes+Caffeine. The Diabetes model was induced by intraperitoneal administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine were administrated 30 minutes before the exercise Stress Test (ST) protocol. During the ST animals underwent a Stress Test (ST), in which they performed forced swimming (until exhaustion) tie to loads of 13% Body’s Weight (BW). The incremental phase of LM began with an initial load of 4% Body’s Weight (BW) and increased 0.5% every 5 min. Lactate concentration was measured 5, 7 and 9 min (mmol/L) after ST. The Incremental Progressive Test (IPT) involved swimming with loads of 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0% of BW, for 5min with each. Blood samples were collected by a caudal puncture to subsequent lactate and hormone assay.

Results: Performance time and lactate concentration of hyperlactatemia test, as well as Lactate Minimum (LM) and Lactate (LAC) concentration after the progressive test presented a significant difference when comparing the levels of the control group with caffeine and diabetic group (p<0.05).

Conclusion: It is suggested that caffeine improves lactate clearance and hormonal steady state condition of diabetic animals after hyperlactacidemia and physical exercise maintenance.

Keywords: Caffeine, conditioning, hyperlactatemia, lactate, metabolism, stress test.

[1]
Roschel H, Tricoli V, Ugrinowitsch C. Physical training: scientific and practical considerations. Braz J Phiscal Educ Sport 2011; 25: 53-65.
[2]
Abreu P, Mendes SV, Leal-Cardoso JH, Ceccatto VM. Anaerobic threshold employed on exercise training prescription and performance assessment for laboratory rodents: a short review. Life Sci 2016; 151: 1-6.
[3]
Gujral UP, Mohan V, Pradeepa R, et al. Ethnic variations in diabetes and prediabetes prevalence and the roles of insulin resistance and β-cell function: the CARRS and NHANES studies. J Clin Transl Endocrinol 2016; 4: 19-27.
[4]
Graham TE. Caffeine and exercise, metabolism, endurance and performance. Sports Med 2001; 31: 785-807.
[5]
Paluska SA. Caffeine and exercise. Curr Sports Med Rep 2003; 2: 213-9.
[6]
Urzúa Z, Trujillo X, Huerta M, et al. Effects of chronic caffeine administration on blood glucose levels and on glucose tolerance in healthy and diabetic rats. J Int Med Res 2012; 40: 2220-30.
[7]
Conde SV, Silva TN, Gonzalez C, Carmo MM, Monteiro EC, Guarino MP. Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats. Br J Nutr 2012; 107: 86-95.
[8]
Guarino MP, Ribeiro MJ, Sacramento JF, Conde SV. Chronic caffeine intake reverses age-induced insulin resistance in the rat: effect on skeletal muscle Glut4 transporters and AMPK activity. Age 2013; 35: 1755-65.
[9]
Egawa T, Hamada T, Kameda N, et al. Caffeine acutely activates 5'adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles. Metabolism 2009; 58: 1609-17.
[10]
Misra M, Aiman U. Alloxan: An unpredictable drug for diabetes induction? Indian J Pharmacol 2012; 44(4): 538-9.
[11]
Araujo GG, Papoti M, Manchado FB, Mello MAR, Gobatto CA. Protocols for hyperlactatemia induction in the lactate minimum test adapted to swimming rats. Comp Biochem Physiol A 2007; 148: 888-92.
[12]
Pallarés JG, Morán-Navarro R, Ortega JF, Fernández-Elías VE, Mora-Rodriguez R. Validity and reliability of ventilatory and blood lactate thresholds in well-trained cyclists. PLoS One 2016; 11e0163389
[13]
Hoff J, Støren Ø, Finstad A, Wang E, Helgerud J. Increased blood lactate level deteriorates running economy in world class endurance athletes. J Strength Cond Res 2016; 30: 1373-8.
[14]
Faude O, Kindermann W, Meyer T. Lactate threshold concepts: how valid are they? Sports Med 2009; 39: 469-90.
[15]
Hansen JS, Zhao X, Irmler M, et al. Type 2 diabetes alters metabolic and transcriptional signatures of glucose and amino acid metabolism during exercise and recovery. Diabetologia 2015; 58: 1845-54.


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Article Details

VOLUME: 15
ISSUE: 6
Year: 2019
Page: [621 - 626]
Pages: 6
DOI: 10.2174/1573401314666180515143951
Price: $65

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