Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

Author(s): Saba Fakhrieh Asl, Mehrnaz Pourvahedi, Ali Mojtahedi*, Mohammad Shenagari.

Journal Name: Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)

Volume 19 , Issue 3 , 2019

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Graphical Abstract:


Abstract:

Objective: Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.

Methods: The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.

Results: Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).

Conclusion: In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

Keywords: Helicobacter pylori, babA, cagE, cagA, gastric disorders, gastric patients.

[1]
Dara, M.; Khashei, R.; Dehghani, B. High frequency of hopQ genotypes among Iranian Helicobacter pylori clinical isolates. Infez. Med., 2017, 25(2), 123-126.
[PMID: 28603230]
[2]
Khashei, R.; Dara, M.; Bazargani, A.; Bagheri Lankarani, K.; Taghavi, A.; Moeini, M.; Dehghani, B.; Sohrabi, M. High rate of A2142G point mutation associated with clarithromycin resistance among Iranian Helicobacter pylori clinical isolates. APMIS, 2016, 124(9), 787-793.
[http://dx.doi.org/10.1111/apm.12567] [PMID: 27357065]
[3]
Nemati, S.; Mojtahedi, A.; Naghavi, S.E.; Banan, R.; Zia, F. Investigating Helicobacter pylori in nasal polyposis using polymerase chain reaction, urease test and culture. Eur. Arch. Otorhinolaryngol., 2012, 269(5), 1457-1461.
[http://dx.doi.org/10.1007/s00405-011-1848-8] [PMID: 22116383]
[4]
Zhang, Z.W.; Dorrell, N.; Wren, B.W.; Farthingt, M.J. Helicobacter pylori adherence to gastric epithelial cells: a role for non-adhesin virulence genes. J. Med. Microbiol., 2002, 51(6), 495-502.
[http://dx.doi.org/10.1099/0022-1317-51-6-495] [PMID: 12018657]
[5]
Kuipers, E.J. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer., 1997.
[http://dx.doi.org/10.1046/j.1365- 2036.11.s1.5.x]
[6]
Wroblewski, L.E.; Peek, R.M., Jr; Wilson, K.T. Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clin. Microbiol. Rev., 2010, 23(4), 713-739.
[http://dx.doi.org/10.1128/CMR.00011-10] [PMID: 20930071]
[7]
Bauer, B.; Meyer, T.F. The Human Gastric Pathogen Helicobacter pylori and Its Association with Gastric Cancer and Ulcer Disease., 2011.
[8]
Costa, A.C.; Figueiredo, C.; Touati, E. Pathogenesis of Helicobacter pylori infection., 2009.
[9]
Kim, S.Y.; Woo, C.W.; Lee, Y.M.; Son, B.R.; Kim, J.W.; Chae, H.B.; Youn, S.J.; Park, S.M. Genotyping CagA, VacA subtype, IceA1, and BabA of Helicobacter pylori isolates from Korean patients, and their association with gastroduodenal diseases. J. Korean Med. Sci., 2001, 16(5), 579-584.
[http://dx.doi.org/10.3346/jkms.2001.16.5.579] [PMID: 11641526]
[10]
Haddadi, M.H.; Bazargani, A.; Khashei, R.; Fattahi, M.R.; Bagheri Lankarani, K.; Moini, M.; Rokni Hosseini, S.M. Different distribution of Helicobacter pylori EPIYA- cagA motifs and dupA genes in the upper gastrointestinal diseases and correlation with clinical outcomes in iranian patients. Gastroenterol. Hepatol. Bed Bench, 2015, 8(Suppl. 1), S37-S46.
[PMID: 26171136]
[11]
Magalhães, A.; Reis, C.A. Helicobacter pylori adhesion to gastric epithelial cells is mediated by glycan receptors. Braz. J. Med. Biol. Res., 2010, 43(7), 611-618.
[http://dx.doi.org/10.1590/S0100-879X2010007500049] [PMID: 20521012]
[12]
Sohrabi, M.; Khashei, R.; Alizadeh, M.; Asl, M.H.; Nejati, M.A.; Dara, M.; Bazargani, A. Low rate of baba2 genotype among iranian helicobacter pylori clinical isolates. J. Clin. Diagn. Res., 2017, 11(7), DC32-DC36.
[http://dx.doi.org/10.7860/JCDR/2017/24810.10277] [PMID: 28892894]
[13]
Con, S.A.; Takeuchi, H.; Nishioka, M.; Morimoto, N.; Sugiura, T.; Yasuda, N.; Con-Wong, R. Clinical relevance of Helicobacter pylori babA2 and babA2/B in Costa Rica and Japan. World J. Gastroenterol., 2010, 16(4), 474-478.
[http://dx.doi.org/10.3748/wjg.v16.i4.474] [PMID: 20101774]
[14]
Ramis, I.B.; Vianna, J.S.; Silva, Junior, L.V.; Von Groll, A.; Silva, P.E. cagE as a biomarker of the pathogenicity of Helicobacter pylori. Rev. Soc. Bras. Med. Trop., 2013, 46(2), 185-189.
[http://dx.doi.org/10.1590/0037-8682-0054-2012] [PMID: 23740068]
[15]
Yamazaki, S.; Yamakawa, A.; Okuda, T.; Ohtani, M.; Suto, H.; Ito, Y.; Yamazaki, Y.; Keida, Y.; Higashi, H.; Hatakeyama, M.; Azuma, T. Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. J. Clin. Microbiol., 2005, 43(8), 3906-3916.
[http://dx.doi.org/10.1128/JCM.43.8.3906-3916.2005] [PMID: 16081930]
[16]
Mitchell, D.J.; Huynh, H.Q.; Ceponis, P.J.; Jones, N.L.; Sherman, P.M. Helicobacter pylori disrupts STAT1-mediated gamma interferon-induced signal transduction in epithelial cells. Infect. Immun., 2004, 72(1), 537-545.
[http://dx.doi.org/10.1128/IAI.72.1.537-545.2004] [PMID: 14688135]
[17]
Day, A.S.; Jones, N.L.; Lynett, J.T.; Jennings, H.A.; Fallone, C.A.; Beech, R.; Sherman, P.M. cagE is a virulence factor associated with Helicobacter pylori-induced duodenal ulceration in children. J. Infect. Dis., 2000, 181(4), 1370-1375.
[http://dx.doi.org/10.1086/315394] [PMID: 10762568]
[18]
Yang, J.J.; Cho, L.Y.; Ko, K.P.; Shin, A.; Ma, S.H.; Choi, B.Y.; Han, D.S.; Song, K.S.; Kim, Y.S.; Lee, J.Y.; Han, B.G.; Chang, S.H.; Shin, H.R.; Kang, D.; Yoo, K.Y.; Park, S.K. Genetic susceptibility on CagA-interacting molecules and gene-environment interaction with phytoestrogens: a putative risk factor for gastric cancer. PLoS One, 2012, 7(2)e31020
[http://dx.doi.org/10.1371/journal.pone.0031020] [PMID: 22383989]
[19]
Eghbali, Z.; Mojtahedi, A.; Moien Ansar, M.; Fakhrieh Asl, S.; Aminian, K. Detection of 23srrna mutations strongly related to clarithromycin resistance in helicobacter pylori strains isolated from patients in the north of Iran. Jundishapur J. Microbiol., 2016, 9(2)e29694
[http://dx.doi.org/10.5812/jjm.29694] [PMID: 27127590]
[20]
Lu, J.J.; Perng, C.L.; Shyu, R.Y.; Chen, C.H.; Lou, Q.; Chong, S.K.; Lee, C.H. Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues. J. Clin. Microbiol., 1999, 37(3), 772-774.
[PMID: 9986850]
[21]
Arévalo-Galvis, A.; Trespalacios-Rangell, A.A.; Otero, W.; Mercado-Reyes, M.M.; Poutou-Piñales, R.A. Prevalence of cagA, vacA, babA2 and iceA genes in H. pylori strains isolated from Colombian patients with functional dyspepsia. Pol. J. Microbiol., 2012, 61(1), 33-40.
[PMID: 22708344]
[22]
Jones, N.L.; Day, A.S.; Jennings, H.A.; Sherman, P.M. Helicobacter pylori induces gastric epithelial cell apoptosis in association with increased Fas receptor expression. Infect. Immun., 1999, 67(8), 4237-4242.
[PMID: 10417197]
[23]
Chomvarin, C.; Namwat, W.; Chaicumpar, K.; Mairiang, P.; Sangchan, A.; Sripa, B.; Tor-Udom, S.; Vilaichone, R.K. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int. J. Infect. Dis., 2008, 12(1), 30-36.
[http://dx.doi.org/10.1016/j.ijid.2007.03.012] [PMID: 17548220]
[24]
Dunne, C.; Dolan, B.; Clyne, M. Factors that mediate colonization of the human stomach by Helicobacter pylori. World J. Gastroenterol., 2014, 20(19), 5610-5624.
[http://dx.doi.org/10.3748/wjg.v20.i19.5610] [PMID: 24914320]
[25]
Maleknejad, S.; Mojtahedi, A.; Safaei-Asl, A.; Taghavi, Z.; Kazemnejad, E. Primary Antibiotic Resistance to Helicobacter pylori Strains Isolated From Children in Northern Iran: A Single Center Study. Iran. J. Pediatr., 2015, 25(6)e2661
[http://dx.doi.org/10.5812/ijp.2661] [PMID: 26635938]
[26]
Das, J.C.; Paul, N. Epidemiology and pathophysiology of Helicobacter pylori infection in children. Indian J. Pediatr., 2007, 74(3), 287-290.
[http://dx.doi.org/10.1007/s12098-007-0046-6] [PMID: 17401270]
[27]
Osman, H.A.; Hasan, H.; Suppian, R.; Hassan, S.; Andee, D.Z.; Abdul Majid, N.; Zilfalil, B.A. Prevalence of Helicobacter pylori cagA, babA2, and dupA genotypes and correlation with clinical outcome in Malaysian patients with dyspepsia. Turk. J. Med. Sci., 2015, 45(4), 940-946.
[http://dx.doi.org/10.3906/sag-1409-77] [PMID: 26422871]
[28]
Ito, Y.; Azuma, T.; Ito, S.; Miyaji, H.; Hirai, M.; Yamazaki, Y.; Sato, F.; Kato, T.; Kohli, Y.; Kuriyama, M. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan. J. Clin. Microbiol., 1997, 35(7), 1710-1714.
[PMID: 9196179]
[29]
Arents, N.L.; van Zwet, A.A.; Thijs, J.C.; Kooistra-Smid, A.M.; van Slochteren, K.R.; Degener, J.E.; Kleibeuker, J.H.; van Doorn, L.J. The importance of vacA, cagA, and iceA genotypes of Helicobacter pylori infection in peptic ulcer disease and gastroesophageal reflux disease. Am. J. Gastroenterol., 2001, 96(9), 2603-2608.
[http://dx.doi.org/10.1111/j.1572-0241.2001.04104.x] [PMID: 11569682]


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VOLUME: 19
ISSUE: 3
Year: 2019
Page: [274 - 278]
Pages: 5
DOI: 10.2174/1871526518666180515113218
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