Objective: Helicobacter pylori is a Gram-negative bacterium which has a serious effect on
up to half of the world’s population and has been related to different gastric diseases. The goal of this
study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated
from gastric biopsies of endoscopic patients in the north of Iran.
Methods: The present study was performed on 90 strains of H. pylori isolated from patients with
gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia
(NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR
method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.
Results: Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%,
30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA
gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2
genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited
8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent
pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).
Conclusion: In summary, as first report from Guilan province in the north of Iran, we showed
significant association between the presence of babA2, cagE, and cagA genes in different types of