Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid

Author(s): Qiang Tan*, Jie Qiu, Xiang Luo, Yewang Zhang, Yuting Liu, Yuquan Chen, Jing Yuan, Wei Liao.

Journal Name: Current Pharmaceutical Biotechnology

Volume 19 , Issue 1 , 2018

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Abstract:

Background: Cephalosporins are the most widely used semisynthetic antibiotics, which acted on bacterial cell wall (peptidoglycan) synthesis. The key intermediate for fabricating about twothirds of cephalosporins in clinical use is 7-aminocephalosporanic acid (7-ACA), which is derived from chemical or enzymatic deacylation of the natural antibiotic cephalosporin C (CPC). The chemical deacylation process has been replaced by the enzymatic deacylation process because the chemical process required harsh conditions and released toxic waste.

Methods: A two-step enzymatic process that utilized D-amino acid oxidase (DAAO) and 7-β-(4- carboxybutanamido)-cephalosporanic acid acylase (GLA) for two successive reactions has been applied for the conversion of CPC to 7-ACA in an industrial scale.

Results: To simplify the process and lower costs, the one-pot enzymatic processes were developed by the application of the mono-enzymatic process (application of cephalosporin C acylase or the variants of GLA), di-enzymatic process (simultaneous action of DAAO and GLA) or the tri-enzymatic process (simultaneous action of DAAO, GLA and catalase) for direct conversion of CPC to 7-ACA.

Conclusion: Here, we mainly focused on the description of these one-pot enzymatic processes and emphasized on the preparation of the involved biocatalysts.

Keywords: One-pot, bioconversion, enzymatic process, cephalosporin C, 7-aminocephalosporanic acid, acylase.

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Article Details

VOLUME: 19
ISSUE: 1
Year: 2018
Page: [30 - 42]
Pages: 13
DOI: 10.2174/1389201019666180509093956

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