The Effect of Sodium Metabisulphite on Apoptosis in the Experimental Model of Parkinson’s Disease

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Author(s): Ayse Ozkan, Hande Parlak, Ozlem Ozsoy, Gamze Tanriover, Sayra Dilmac, Eylem Turgut, Piraye Yargicoglu, Aysel Agar*.

Journal Name: Current Nutrition & Food Science

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Background: The aim of this study was to investigate the mechanisms underlying possible toxic effects of sulphite on neurodegeneration.

Methods: Male Wistar rats were assigned to each of the four groups: Control (Control), Sulphitetreated (Sulphite), 6-hydroxydopamine (6-OHDA)-injected (6-OHDA), and sulphite-treated and 6- OHDA-injected (6-OHDA+Sulphite). Sodium metabisulphite was administered orally by gavage at a dose of 100 mg/kg/day for 45 days. Experimental PD was created stereotactically via the unilateral infusion of 6-OHDA into the medial forebrain bundle (MFB). Rotarod performances, plasma Ssulfonate levels, caspase-3 activities, Bax and Bcl-2 levels, tyrosine hydroxylase (TH) and cleaved caspase-3 double staining were investigated.

Results: The rotarod test showed that the 6-OHDA-injected animals exhibited shorter time on the rod mile compared to the control group; however, there was no difference between 6-OHDA and 6- OHDA+Sulphite groups. Plasma levels of S-sulfonate in Sulphite and 6-OHDA+ Sulphite groups increased in contrast to their corresponding control groups. Caspase-3 enzyme activity increased in the 6-OHDA group whereas it did not in control. However, sulphite treatment did not affect these activity levels. Anti-apoptotic protein Bcl-2 concentration decreased, but the concentration of pro-apoptotic protein Bax increased in the 6-OHDA group compared to the control group. The expression of caspase-3 increased, while the number of tyrosine hydroxylase (TH)-positive neurons decreased in 6- OHDA group as compared to the control groups. However, sulphite treatment had no effect on these parameters.

Conclusion: Sulphite is not a potentially aggravating factor for the activity of caspase-3 in a 6- OHDA-induced experimental model of Parkinson’s disease.

Keywords: Parkinson`s Disease, Sulphite, Bax, Bcl-2, Caspase-3

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(E-pub Ahead of Print)
DOI: 10.2174/1573401314666180503153444
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