The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

Author(s): Paula Sofia Coutinho Medeiros, Ana Lúcia Marques Batista de Carvalho*, Cristina Ruano, Juan Carlos Otero, Maria Paula Matos Marques.

Journal Name: Letters in Drug Design & Discovery

Volume 16 , Issue 2 , 2019

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Abstract:

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells.

Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line.

Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability.

Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

Keywords: Dietary antioxidants, p-coumaric acid, breast cancer cells, chemoprotective/chemotherapeutic, raman microspectroscopy, antitumor activities.

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VOLUME: 16
ISSUE: 2
Year: 2019
Page: [127 - 137]
Pages: 11
DOI: 10.2174/1570180815666180502120804
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