Objective: The present research was designed to appraise the cardioprotective activity of a
hydroalcoholic extract of Agaricus bisporus (EEAB) on Isoproterenol (ISO) induced myocardial infarction
(MI) in Albino Wistar rat. Traditionally, Agaricus bisporus is reported in the treatment of
heart diseases, cancer, cerebral stroke and anti-ageing property.
Material and Method: Wistar rats of different sex were randomly split into five groups namely positive
control, negative control, standard, test-1 and test-2 and received distilled water, ISO (85 mg/kg),
Simvastatin (10 mg/kg/day, oral) and EEAB (200 and 400 mg/kg/day, p.o.) for 30 days, respectively.
MI was induced in rats by ISO at an interval of 24 hrs on 31 and 32 day and on the next day, blood
was amassed through retro-orbital plexus for the assessment of biochemical markers (cholesterol, lowdensity
lipoprotein, high-density lipoprotein, very low-density lipoprotein, triglycerides, alanine aminotransferase
and total protein) and finally, the rats were immolated by cervical dislocation. The heart
tissue was reaped instantly, cleaned with chilled isotonic saline and clasped in 10% buffered formalin
and used for the histopathological analysis.
Results: ISO p.o. administration significantly elevated the cholesterol, low density lipoprotein, very
low density lipoprotein, triglycerides, alanine aminotransferase and aspartate aminotransferase levels
while it decreases high-density lipoprotein and total protein in plasma and administration of EEAB
decreases the level of cholesterol, low-density lipoprotein, very low-density lipoprotein, triglycerides,
alanine aminotransferase and aspartate aminotransferase levels while it increases high-density lipoprotein
and total protein levels. Pretreatment with EEAB protected the cardiotoxicity induced by ISO.
The histopathological findings support the analysis of biochemical parameters, ISO-induced myocardium
showed infracted zone with edema, inflammatory cells, lipid droplets, myocardial necrosis and
vacuolization of myofibrils which were reduced.
Conclusion: It can be an outcome that EEAB possessed cardioprotective activity against experimental
and clinical studies of ISO-induced myocardial infarction in rats.