Background: Adipose tissue is one of the most attractive sources of stem cells because it
can be easily harvested and yields a greater stromal cell density. The multilineage potential of adiposederived
stem cells (ADSCs) demonstrates their significant impact within the field of tissue engineering,
with studies successfully demonstrating the ability to produce a range of tissue types. However,
although a broad spectrum of applications has already been suggested, many important scientific and
medical questions remain unanswered before the clinical application of ADSCs in humans. Importantly,
clarification of the biology and identification of the differences of ADSCs from various areas of
the body is required. In this continuous endeavor, research in rat models plays an important role in the
development of new knowledge.
Methods: A literature review was done to summarize all information regarding harvesting, isolation,
expansion, cryopreservation and differentiation of rat ADSCs. A Wistar rat model was also used to describe
harvesting sites of adipose tissue, and to characterize the ADSCs using Fourier-transform infrared
(FT-IR) spectroscopy and phase contrast microscopy.
Aims: To discuss all relevant considerations for harvesting, culture, differentiation and phenotypic
characterization of ADSCs, to provide a comprehensive roadmap of this process, to identify the differences
between ADSCs obtained from various adipose tissues of the rat, and to provide FT-IR spectroscopy
marker bands that could be used as fingerprints to differentiate the types of adipose tissues.