Background: Many health hazardous diseases are caused by clinical pathogens. Drugresistant
microbes are one of the major health problems in the world. To overcome the effect of infectious
diseases new antimicrobial agent from nature has been explored which is environmentally
friendly, less costly and more effective for the development of next-generation drugs. Bergenia ciliata
and silver nitrate both have medicinal properties.
Objectives: The aim of the current research was to evaluate the cytotoxic, and antibacterial effect of
green synthesized nanoparticles using Bergenia ciliata rhizome against clinical bacterial pathogens.
Methods: Extract of Bergenia ciliata was prepared by maceration technique. Silver nanoparticles were
synthesized using Bergenia ciliata rhizome extract. Synthesized silver nanoparticles were confirmed
by UV-vis spectrophotometer, Scanning electron microscope (SEM) and Fourier Transform Infrared
Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, DNA protection,
brine shrimp lethality effects of synthesized nanoparticles were investigated.
Results: UV-vis spectrophotometer indicated the prelaminar synthesis of silver nanoparticles at 400
nm. The spherical shape of synthesized nanoparticles with 35 nm size was confirmed using SEM.
Greatest zone of inhibition (6.0 ± 0.0 mm to 8.3 ± 0.57 mm) was recorded against all tested pathogens
compared with the B. ciliata aqueous extract. Anti-biofilm analysis and MTT assay supported the results
of the antibacterial activity. Silver nanoparticles protect the DNA degradation.
Conclusion: Green synthesized nanoparticles had potent antibacterial activity and may provide a basis
for the development of the new antibacterial drug.