In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests

Author(s): Zehao Huang, Na Li, Kaifeng Rao, Cuiting Liu, Zijian Wang*, Mei Ma*.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 21 , Issue 4 , 2018

Abstract:

Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.

Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China.

Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549.

Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

Keywords: SOS/umu tests, high-content screening, in vitro micronucleus tests, cytotoxicity, genotoxicity, tannery chemicals.

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Article Details

VOLUME: 21
ISSUE: 4
Year: 2018
Page: [262 - 270]
Pages: 9
DOI: 10.2174/1386207321666180330120248
Price: $58

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