Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells

Author(s): Xiaofen Ni, Jiajun Chen, Fengying Lu, Zhengzhong Yuan, Xiaofeng Xu, Zhen Hu*, Lei Jiang*.

Journal Name: Recent Patents on Anti-Cancer Drug Discovery

Volume 13 , Issue 2 , 2018

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Abstract:

Background: -Solanine, the most important and active component of Solanum nigrum, was found to have anti-cancer activity on multiple cancer cells. However, its effects on colorectal cancer (CRC) and associated molecular mechanisms remain to be further elucidated.

Objective: In this study, we investigated the anti-cancer effects of α-solanine against CRC cells in vitro and in vivo.

Materials & Methods: Cell viability was measured using Cell Counting Kit-8 (CCK-8) assay; cell cycle was analyzed with a Cycletest Plus DNA Reagent Kit; cell apoptosis was detected by flow cytometer; cell migration and invasive ability was determined by Transwell assays; S100P protein expression was also analyzed by western blotting; lentiviral vectors expressing shRNA targeting the S100P gene.

Results: We demonstrated that α-solanine inhibited CRC cell (SW480, SW620 and HT-29) growth as well as migration and invasion, induced cell cycle arrest and apoptosis in vitro, and suppressed tumor growth in vivo. Moreover, we observed that S100P expression was downregulated by α-solanine. Overexpression of S100P partially reversed the α-solanine-induced growth inhibition of CRC cells. Conversely, knockdown of S100P by lentiviral-mediated RNAi resulted in significantly promoting the α-solanine-induced growth inhibition.

Conclusion: These findings suggest that α-solanine is a potential agent for the treatment of CRC, and the anti-tumor effect of α-solanine in the CRC cells may be mediated at least partly by the downregulation of S100P.

Keywords: Anti-tumor effect, apoptosis, cell-cycle arrest, colorectal cancer, invasion, migration, S100P, α-solanine.

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Article Details

VOLUME: 13
ISSUE: 2
Year: 2018
Page: [240 - 247]
Pages: 8
DOI: 10.2174/1574892813666180329163945
Price: $58

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