Functionalized Multi Walled Carbon Nanotubes-Reinforced Hollow Fiber Solid/Liquid Phase Microextraction and HPLC-DAD for Determination of Phenazopyridine in Urine

Author(s): Nabil N. Al-Hashimi*, Anas I. Awwad, Aqeel N. Al-Hashimi, Iman A. Mansi, Rand O. Shahin, Saja H. Hamed.

Journal Name: Current Pharmaceutical Analysis

Volume 15 , Issue 5 , 2019

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Introduction: A sensitive analytical method based on functionalized multi walled carbon nanotubes reinforced hollow fiber solid/liquid phase microextraction (F-MWCNTs-HF-SLPME) forwarded with HPLC-DAD for analyzing phenazopyridine from urine is presented.

Materials and Methods: The extraction of phenazopyridine is performed using specially designed FMWCNTs- HF-SLPME device constructed as follows: the functionalized multi walled carbon nanotubes (F-MWCNTs) were immobilized into the pores of 2.5 cm hollow fiber micro-tube using capillary forces and ultrasonication, then, the lumen of the micro-tube was filled with 1-octanol with two ends sealed. Subsequently, the device was placed into 10-mL of urine sample containing the analyte with agitation. After ending extraction, the device was removed, rinsed, sonicated in 250 µL of organic solvent and analyzed directly by the separation system.

Conclusion: Different parameters affecting the performance of the developed method were optimized. The method showed good linearity with (R2) 0.999 and good repeatability with (RSDs) from 3.7 to 0.9% at analyte concentration ranged from 0.01 to 10 µg L-1 of spiked urine samples. The limit of detection/ limits of detection quantitation LODs/LOQs was 0.02/0.09 µg L-1. In comparison with reference methods, the developed method is considered as a promising microextraction technique for determination of trace phenazopyridine in human urine using a common HPLC without further cleanup procedures

Keywords: Functionalized multi-walled carbon nanotubes, liquid chromatography, phenazopyridine, reinforced solid/liquid phase microextraction, urine, HPLC-DAD.

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Article Details

Year: 2019
Page: [447 - 455]
Pages: 9
DOI: 10.2174/1573412914666180329153443
Price: $95

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