Identification of Moesin as a Novel Autoantigen in Patients with Sjögren’s Syndrome

Author(s): Yixuan Zhang, Muhammad Hussain, Xiuqin Yang, Peng Chen, Chunhe Yang, Yiping Xun, Yaping Tian*, Hongwu Du*.

Journal Name: Protein & Peptide Letters

Volume 25 , Issue 4 , 2018

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Abstract:

Background: SjOgren's Syndrome (SS) is a systemic and chronic autoimmune disorder that affects the exocrine glands with massive autoantibody production. Although the pathogenesis of the disorder is incompletely understood, but some studies have reported that anti-moesin antibodies have been detected in autoimmune diseases with which SS is closely associated. Here, we have investigated moesin's potential involvement in SS.

Objective: This study aims to verify whether moesin is a specific autoantigen involved in Chinese Hans SS patients.

Methods: First, recombinant human moesin was expressed and purified. Next, the protein was verified as antigen by Western blotting and immunoprecipitation. The positive protein band in the immunoprecipitation was identified by (MALDI-TOF/TOF). Finally, an optimized ELISA (Enzyme- Linked Immunosorbent Assay) kit was developed to measure the titer concentration of anti-moesin antibody-positive patients in a large cohort of clinical subjects.

Results: Univariate analysis revealed that the proportion of individuals positive for serum IgG against recombinant human moesin was 42 % in a group of Chinese Hans SS patients (21 of 50), 22 % in systemic lupus erythematosus patients and (11 of 50), compared to only 4 % in healthy controls (2 of 50).

Conclusion: An association between anti-moesin antibodies and SS manifestation have been found which may be considered a suspected serum biomarker for the development of SS.

Keywords: Autoantibody, autoantigen, moesin, Sjögren’s syndrome, autoimmune disorder, anti-moesin antibodies.

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Article Details

VOLUME: 25
ISSUE: 4
Year: 2018
Page: [350 - 355]
Pages: 6
DOI: 10.2174/0929866525666180320110135
Price: $58

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