Identification of Moesin as a Novel Autoantigen in Patients with Sjögren’s Syndrome

Title:Identification of Moesin as a Novel Autoantigen in Patients with Sjögren’s Syndrome

Volume: 25 Issue: 4

Author(s): Yixuan Zhang, Muhammad Hussain, Xiuqin Yang, Peng Chen, Chunhe Yang, Yiping Xun, Yaping Tian*Hongwu Du*

Affiliation:

• Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853,China

• 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083,China

Keywords: Autoantibody, autoantigen, moesin, Sjögren’s syndrome, autoimmune disorder, anti-moesin antibodies.

Abstract: Background: SjOgren's Syndrome (SS) is a systemic and chronic autoimmune disorder that affects the exocrine glands with massive autoantibody production. Although the pathogenesis of the disorder is incompletely understood, but some studies have reported that anti-moesin antibodies have been detected in autoimmune diseases with which SS is closely associated. Here, we have investigated moesin's potential involvement in SS.

Objective: This study aims to verify whether moesin is a specific autoantigen involved in Chinese Hans SS patients.

Methods: First, recombinant human moesin was expressed and purified. Next, the protein was verified as antigen by Western blotting and immunoprecipitation. The positive protein band in the immunoprecipitation was identified by (MALDI-TOF/TOF). Finally, an optimized ELISA (Enzyme- Linked Immunosorbent Assay) kit was developed to measure the titer concentration of anti-moesin antibody-positive patients in a large cohort of clinical subjects.

Results: Univariate analysis revealed that the proportion of individuals positive for serum IgG against recombinant human moesin was 42 % in a group of Chinese Hans SS patients (21 of 50), 22 % in systemic lupus erythematosus patients and (11 of 50), compared to only 4 % in healthy controls (2 of 50).

Conclusion: An association between anti-moesin antibodies and SS manifestation have been found which may be considered a suspected serum biomarker for the development of SS.

Cite this article as:

Zhang Yixuan , Hussain Muhammad , Yang Xiuqin , Chen Peng , Yang Chunhe , Xun Yiping , Tian Yaping*, Du Hongwu *, Identification of Moesin as a Novel Autoantigen in Patients with Sjögren’s Syndrome, Protein & Peptide Letters 2018; 25 (4) . https://dx.doi.org/10.2174/0929866525666180320110135