Objective: To evaluate the hypolipidemic and antioxidant effects of aqueous
extract leaves of Anvillea radiata (AEL of A. radiata) in normal and streptozotocin (STZ)
induced diabetic rats. In addition, the determination of total polyphenol and total flavonoid
contents in the AEL of A. radiate was performed.
Methods: The effects of oral administration of AEL of A. Radiata (10 mg/kg) on the
plasma total cholesterol (TC), triglyceride (TG) and high-density lipoprotein-cholesterol
(HDL-C) concentrations were measured in both normal and diabetic rats. The antioxidant
capacity was realized by the method of DPPH. Total polyphenol, as well as, flavonoid
contents of the AEL of A. radiata were determined by Folin-Ciocalteu method and colorimetric
AlCl3 method, respectively.
Results: AEL of A. radiate (10 mg/kg) showed a strong hypolipidemic effect both in
normoglycaemic and in STZ rats after 15 days of daily treatment. In normal rats, AEL of
A. radiata induced a significant decrease in plasma triglyceride levels (p<0.0001).
Moreover, plasma cholesterol total levels reduced significantly (p<0.05; p<0.0001) both
in normal and diabetic rats, respectively. In contrast, plasma HDL-C levels showed a significant
increase (p<0.05; p<0.01) both in normal and in STZ induced diabetic rats, respectively.
On the other side, AEL of A. radiata showed important antioxidant activity
and revealed the inhibitory concentration of 50% of free radicals (IC50) (IC50=343.41
mg/ml). Concerning the quantitative determination of total polyphenol and flavonoid contents,
which were equal to 70.28 mg of gallic acid equivalent per gram of extract (mg
EAG/g) and 16.38 mg of quercetin equivalent per gram of extract (EQ/g), respectively.
Conclusion: AEL of A. radiata (10 mg/kg) shows the potential hypolipidemic and antioxidant
effects in both normal and STZ-induced diabetic rats.