Blood microRNA-15a Correlates with IL-6, IGF-1 and Acute Cerebral Ischemia

Author(s): Wen-Jing Lu , Li-Li Zeng* , Yang Wang , Yu Zhang , Huai-Bin Liang , Xuan-Qiang Tu , Ji-Rong He , Guo-Yuan Yang .

Journal Name: Current Neurovascular Research

Volume 15 , Issue 1 , 2018

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Abstract:

Objective: This study aims to explore the function of blood microRNA-15a (miR-15a) in the pathogenesis of acute cerebral ischemia (AIS).

Methods: Blood samples were collected from healthy control and AIS patients within 72 h after onset. A model of ischemia in human umbilical vein endothelial cells (HUVECs) was established through oxygen and glucose deprivation (OGD). MiR-15a in patients and in cells was measured using real-time quantitative polymerase chain reaction (qPCR). The predicted target of miR-15a such as interleukin-6 (IL-6) and insulin-like growth factors-1 (IGF-1) in plasma was detected by enzyme-linked immunosorbent assay (ELISA). The relations between blood miR-15a and stroke severity, stroke etiology, infarct location, stroke prognosis, predicted targets were analyzed by Statistical Product and Service Solutions (SPSS) software respectively.

Results: Higher miR-15a levels were found in AIS patients and ischemic cells within 72 h, compared to control (p < 0.05). Receiver Operating Characteristic (ROC) analysis showed that blood miR-15a predicted stroke onset with 98.67% specificity. Blood miR-15a had a negative correlation with National Institutes of Health Stroke Scale (NIHSS) scores (r = -0.3695, p < 0.01). The AIS patients with increased miR-15a levels had a better prognosis. MiR-15a was up-regulated in anterior circulation infarction and small-artery atherosclerosis stroke. Plasma levels of IL-6 and IGF-1 were associated with blood miR-15a (r = -0.6051, 0.3231, p < 0.05, respectively).

Conclusion: Blood miR-15a associates with IL-6, IGF-1 and acute cerebral ischemia. It could serve as a potential diagnostic biomarker and therapeutic target for stroke.

Keywords: Human Umbilical Vein Endothelial Cells (HUVECs), Oxygen and Glucose Deprivation (OGD), Interleukin-6 (IL- 6), Insulin-like Growth Factors-1 (IGF-1), microRNA-15a (miR-15a), stroke.

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Article Details

VOLUME: 15
ISSUE: 1
Year: 2018
Page: [63 - 71]
Pages: 9
DOI: 10.2174/1567202615666180319143509
Price: $58

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