Background & Objective: Aging is characterized by gradual accumulation of macromolecular
damage leading to progressive loss of physiological function and increased susceptibility to diverse
diseases. Effective anti-aging strategies involving caloric restriction or antioxidant supplementation
are receiving growing attention to attenuate macromolecular damage in age associated pathology.
Method: In the present study, we for the first time investigated the effect of quercetin, caloric restriction
and combined treatment (caloric restriction with quercetin) on oxidative stress parameters, acetylcholinesterase
and ATPases enzyme activities in the cerebral cortex of aged male Wistar rats. 21
months aged rats were divided into four groups (n=6-8) such as group 1-fed ad libitum (AL); group
2-quercetin supplementation of 50 mg/kg b.w/day for 45 days fed ad libitum (QUER); group 3: caloric
restricted (CR) (fed 40% reduced AL for 45 days); group 4-fed 40% CR and 50 mg/kg b.w/day QUER
for 45 days (CR + QUER). Group 5-three month age old rats served as young control (YOUNG).
Results: Our results demonstrate that combined treatment of caloric restriction and quercetin significantly
improved the age associated decline in the activities of endogenous antioxidant enzymes [such
as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)] and glutathione
(GSH) content and attenuated elevated levels of protein carbonyl content (PCC), lipid peroxidation,
lipofuscin, reactive oxygen species (ROS), and nitric oxide (NO). Furthermore, it is also observed that
combined treatment ameliorated age associated alterations in acetylcholine esterase (AChE) and
adenosine triphosphatases (ATPases) such as Na+/K+-ATPase and Ca+2-ATPase (but not Mg+2-
ATPase) enzyme activities.
Conclusion: Finally, we conclude that combined treatment of caloric restriction and quercetin (but not
either treatment alone) in late life is an effective anti-aging therapy to counteract the age related accumulation
of oxidative macromolecular damage.