Hypnotic Effect of Portulaca oleracea on Pentobarbital-Induced Sleep in Mice

Author(s): Shokouhsadat Hamedi, Fatemeh Forouzanfar, Hassan Rakhshandeh*, Amirali Arian.

Journal Name: Current Drug Discovery Technologies

Volume 16 , Issue 2 , 2019

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Graphical Abstract:


Abstract:

Objective: In Iranian Traditional Medicine, the herbs with cold and wet temperament can help to improve insomnia. Portulaca oleracea has a cold and wet temperament, so the present study was carried out to investigate the sleep-prolonging effect of Portulaca oleracea.

Methods: This work was an experimental study on mice which were randomly divided into these groups: saline (control); Diazepam: positive control); hydro-alcoholic extract of Portulaca oleracea (12.5, 25, 50, 75 and 100 mg/kg) by Soxhlet apparatus and maceration; in the effective (dose25 mg/kg), different fractions of extract were tested. Ethyl acetate fraction (EAF:); N hexane fraction (n-HF); water fraction (WF). All the test compounds were injected intraperitoneally (IP) 30 minutes before pentobarbital administration (30 mg/kg). Duration and latency of pentobarbital-induced sleep were recorded. Also, LD50 of Portulaca oleracea extract was determined and the possible neurotoxicity of the extract was tested on neural PC12 cells. Besides, 30 min after administration of hydroalcoholic extract (HAE) motor coordination (rota-rod test) was assessed.

Results: HAE increased the duration of pentobarbital-induced sleep at doses of 25, 50, 75 and 100 mg/kg. The hypnotic effect of HAE was comparable to that induced by diazepam. Similarly, WF, EAF, and NHF at 25 mg/kg could increase sleep duration. The sleep latency was decreased by HAE and NHF but not by WF and EAF. The LD50 value for HAE was found to be 4.8 g/Kg. HAE and its fractions did not show neurotoxic effect in cultured PC12-cell line, also HAE did not affect the animals performance on the rotarod test.

Conclusion: The present data demonstrated that Portulaca oleracea potentiates sleeping behaviors. The main components responsible for the hypnotic effects of this plant is most likely a non-polar agents which is found in NHF. Isolation of the active constituents may yield a novel sedative drug.

Keywords: Pentobarbital, Portulaca oleracea, sleep, Insomnia, hypnotic effects, N hexane fraction.

[1]
Feyzabadi Z, Jafari F, Feizabadi PS, Ashayeri H, Esfahani MM. Badiee Aval Sh. Insomnia in Iranian Traditional Medicine. Iran Red Crescent Med J 2014; 16(3): e15981.
[2]
Zaharna M, Guilleminault C. Sleep, noise and health.: Review. Noise Health 2010; 12(47): 64-9.
[3]
Orzeł-Gryglewska J. Consequences of sleep deprivation. Int J Occup Med Environ Health 2010; 23(1): 95-114.
[4]
Uzun S, Kozumplik O, Jakovljević M, Sedić B. Side effects of treatment with benzodiazepines. Psychiatr Danub 2010; 22(1): 90-3.
[5]
Noras MR, Yousefi M, Kiani MA. Complementary and alternative medicine (CAM) Use in pediatric disease: A short review. Int J Pediatr 2013; 1(2): 45-9.
[6]
Z Q. Traditional. Medicine: Definitions". Geneva 2008 [updated 2014].
[7]
Ershadifar T, Minaiee B, Gharooni M, et al. Heart palpitation from traditional and modern medicine perspectives. Iran Red Crescent Med J 2014; 16(2): e14301.
[8]
MH A Makhzan-AL Advieh [Farsi]. Tehran, Iran: Tehran University of Medical Science Press 2011; p. 230.
[9]
Avicenna Canon of Medicine [Farsi]. Tehran, Iran: Soroosh Publisher 1986; p. 373.
[10]
H M. Maaref Giyahi. (Plant Sciences). Tehran, Iran: Islamic culture publisher; 2009.
[11]
Organization UNID, Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction technologies for medicinal and aromatic plants: Earth. Environmental and Marine Sciences and Technologies 2008.
[12]
Askari VR, Rahimi VB, Ghorbani A, Rakhshandeh H. Hypnotic Effect of Ocimum basilicum on Pentobarbital-Induced Sleep in Mice. Iran Red Crescent Med J 2016; 18(7): e24261.
[13]
Mortazavian SM, Ghorbani A. Antiproliferative effect of Viola tricolor on neuroblastoma cells in vitro. Aust J Herb Med 2012; 24(3): 93.
[14]
Mortazavian S, Ghorbani A, Hesari TG. Effect of hydro-alcoholic extract of Viola tricolor and its fractions on proliferation of uterine cervix carcinoma cells. Iran J Obst Gyncol Infertil 2012; 15: 9-16.
[15]
Rakhshandah H, Shakeri MT, Ghasemzadeh MR. Comparative hypnotic effect of Rosa damascena fractions and Diazepam in Mice. Iran J Pharm Res 2010; 6(3): 193-7.
[16]
Hosseini A, Ghorbani A, Sadeghnia HR, Rajabian A, Rakhshandeh H. Potentiating effects of Lactuca serriola on pentobarbital-induced sleep. Iran J Pharm Res 2013; 12(2): 401-6.
[17]
Rakhshandeh H, Sadeghnia HR, Ghorbani A. Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice. Nat Prod Res 2012; 26(22): 2095-8.
[18]
Perazzo FF, Carvalho J, Carvalho J, Rehder V. Central properties of the essential oil and the crude ethanol extract from aerial parts of Artemisia annua L. Pharmacol Res 2003; 48(5): 497-502.
[19]
Vafaee F, Hosseini M, Sadeghnia HR, Hadjzadeh MA-r, Soukhtanloo M, Rahimi M. The effects of soy extract on spatial learning and memory damage induced by global ischemia in ovariectomised rats. Malays J Med Sci 2014; 21(3): 19-30.
[20]
Forouzanfar F, Ghorbani A, Rakhshandeh H, Hosseini M. Hydroalcoholic extract of needles of Pinus eldarica enhances pentobarbital-induced sleep: possible involvement of GABAergic system. Avicenna J Phytomed 2016; 6(4): 449-57.
[21]
Shetty Akhila J, Alwar M. Acute toxicity studies and determination of median lethal dose. Curr Sci 2007; 93: 917-20.
[22]
Ghorbani A, Rakhshandeh H, Sadeghnia H. Potentiating effects of Lactuca sativa on pentobarbital-induced sleep. Iran J Pharm Res 2013; 12(2): 401-6.
[23]
Huang F, Xiong Y, Xu L, Ma S, Dou C. Sedative and hypnotic activities of the ethanol fraction from Fructus Schisandrae in mice and rats. J Ethnopharmacol 2007; 110(3): 471-5.
[24]
Herrera-Ruiz M, Gutiérrez C, Jiménez-Ferrer JE, Tortoriello J, Mirón G, León I. Central nervous system depressant activity of an ethyl acetate extract from Ipomoea stans roots. J Ethnopharmacol 2007; 112(2): 243-7.
[25]
Alnamer R, Alaoui K, Bouidida EH, Benjouad A, Cherrah Y. Sedative and hypnotic activities of the methanolic and aqueous extracts of Lavandula officinalis from Morocco. Adv Pharmacol Sci 2012; 2012: 270824.
[26]
Rakhshandah H, Hosseini M, Dolati K. Hypnotic effect of Rosa damascena in mice. Iran J Pharm Res 2010; 181-5.
[27]
Sultana A, Rahman K. Portulaca oleracea Linn. A global Panacea with ethno-medicinal and pharmacological potential. Int J Pharm Pharm Sci 2013; 5: 33-9.
[28]
Rakotonirina VS, Bum EN, Rakotonirina A, Bopelet M. Sedative properties of the decoction of the rhizome of Cyperus articulatus. Fitoterapia 2001; 72(1): 22-9.
[29]
Paredes SD, Korkmaz A, Manchester LC, Tan D-X, Reiter RJ. Phytomelatonin: a review. J Exp Bot 2009; 60(1): 57-69.


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Article Details

VOLUME: 16
ISSUE: 2
Year: 2019
Page: [198 - 203]
Pages: 6
DOI: 10.2174/1570163815666180308142543
Price: $65

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