Purification is a key step for different types of approaches, ranging from food, biotechnology
to pharmaceutical fields. In biotechnology, tangential flow filtration (TFF) allows obtaining the
separation of different components of cells without instability phenomena. In the food industry, TFF
ensures the removal of contaminants or other substances that negatively affect visual appearance, organoleptic
attributes, nutritional value and/or safety of ailments.
In the pharmaceutical area, purification is also an important and necessary step controlling the quality
of final product. In the field of research and development of nanomedicines, several techniques are
used to purify and/or to concentrate the batches for in vitro and in vivo applications. Despite many approaches
that exist; current data reveal continued unsatisfactory results. Between them, TFF showed
promising results, even if, currently, its use is uncommon if compared with other purification techniques
usually reported in “materials and methods” sections.
This review represents an overview of the different applications of TFF from protein purification to
food application, with particular attention to the field of nanomedicine from polymeric to metallic
nanoparticles, highlighting advantages and dis-advantages in the use of this technique. The theoretical
aspect of the process has been examined.