Abstract
Background: Gene therapy is an expanding field and it can treat genetic and acquired diseases.
Objective: It was found that formulations with DNA: CM-β–CD (Carboxymethyl-beta-cyclodextrin): Pluronic-F127 1:3:3 and 1:3 DNA: CM-β–CD are the most stable formulations indicating high incorporation of DNA within CM-β –CD.
Method: Gel electrophoresis revealed DNA with low CM-β –CD concentration has formed a more stable complex. Samples 1:3 DNA: CM-β–CD and 1:3:3 DNA: CM-β–CD: Pluronic-127 show no DNA fragment, suggesting good condensation of DNA inside cyclodextrin cavity.
Results: This was confirmed by fluorescence data where fluorescence intensity was reduced for samples DNA: CM-β–CD 1:3. Overall, the findings showed that Carboxymethyl-beta-cyclodextrin (as a novel non-viral gene vector) was able to provide condensation and protection to the DNA, with and without Pluronic-F127, at low concentration.
Conclusion: pDNA/CM-β–CD complex has not only shown to be able to transfect COS 7 and SHSY5Y cell lines, but it gives a higher transfection efficiency than that produced by the TransIT-LT1 commercial transfection reagent.
Keywords: pDNA, Carboxymethyl-β-cyclodextrin, freeze drying, transfection, gel electrophoresis, gene therapy.
Current Drug Delivery
Title:Insights into the Influences of Carboxymethyl-β-Cyclodextrin on DNA Formulations Characteristics and Gene Transfection Efficiency
Volume: 15 Issue: 6
Author(s): Hassan Elsana, Svetlana Mysina, Eman Ali Elkordy, Jane Carr-wilkinson and Amal Ali Elkordy*
Affiliation:
- University of Sunderland, Sunderland,United Kingdom
Keywords: pDNA, Carboxymethyl-β-cyclodextrin, freeze drying, transfection, gel electrophoresis, gene therapy.
Abstract: Background: Gene therapy is an expanding field and it can treat genetic and acquired diseases.
Objective: It was found that formulations with DNA: CM-β–CD (Carboxymethyl-beta-cyclodextrin): Pluronic-F127 1:3:3 and 1:3 DNA: CM-β–CD are the most stable formulations indicating high incorporation of DNA within CM-β –CD.
Method: Gel electrophoresis revealed DNA with low CM-β –CD concentration has formed a more stable complex. Samples 1:3 DNA: CM-β–CD and 1:3:3 DNA: CM-β–CD: Pluronic-127 show no DNA fragment, suggesting good condensation of DNA inside cyclodextrin cavity.
Results: This was confirmed by fluorescence data where fluorescence intensity was reduced for samples DNA: CM-β–CD 1:3. Overall, the findings showed that Carboxymethyl-beta-cyclodextrin (as a novel non-viral gene vector) was able to provide condensation and protection to the DNA, with and without Pluronic-F127, at low concentration.
Conclusion: pDNA/CM-β–CD complex has not only shown to be able to transfect COS 7 and SHSY5Y cell lines, but it gives a higher transfection efficiency than that produced by the TransIT-LT1 commercial transfection reagent.
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Cite this article as:
Elsana Hassan , Mysina Svetlana , Elkordy Ali Eman, Carr-wilkinson Jane and Elkordy Ali Amal *, Insights into the Influences of Carboxymethyl-β-Cyclodextrin on DNA Formulations Characteristics and Gene Transfection Efficiency, Current Drug Delivery 2018; 15 (6) . https://dx.doi.org/10.2174/1567201815666180226115503
DOI https://dx.doi.org/10.2174/1567201815666180226115503 |
Print ISSN 1567-2018 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5704 |
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