In Vitro Estimation of Photo-Protective Potential of Pomegranate Seed Oil and Development of a Nanoformulation

Author(s): Surbhi Dhawan, Sanju Nanda*.

Journal Name: Current Nutrition & Food Science

Volume 15 , Issue 1 , 2019

Submit Manuscript
Submit Proposal

Graphical Abstract:


Background: Since ancient times, people have been using natural resources for photoprotection purposes. One such highly recognised natural agent is pomegranate seed oil, considered as wonder oil owing to the presence of several beneficial phytoconstituents.

Objective: The study aimed to establish the photoprotective potential of pomegranate seed oil through various in vitro and biochemical studies along with the formation of nanoemulsion, an efficient topical delivery system for the oil.

Method: Photo-protective potential of the oil was estimated by determining in vitro antioxidant and anti-inflammatory activity, total phenolic content, anti elastase, antihyaluronidase and anticollagenase activities of the oil. Ultrasonication method was used to formulate nanoemulsions. The optimisation was done following the central composite design. The characterisation was done by particle size analysis, zeta potential, polydispersity index, pH, viscosity, stability testing and transmission electron microscopy. The optimised nanoemulsion was loaded into a gel base for topical application and further release studies were carried out.

Results: The IC50 values of anti-elastase, anti-collagenase and anti-hyaluronidase were found to be 309 mg/ml, 4 mg/ml and 95 mg/ml respectively. The results of anti-oxidant and anti-inflammatory activity were also significant, which thereby established the photo-protective potential of the oil. The optimum batch 2 had particle size 83.90 nm, 0.237 PDI and -5.37 mV zeta potential. The morphology was confirmed by TEM. Batch 2 was incorporated into a gel base and release studies showed 74.12 % release within 7 hours.

Conclusion: Pomegranate seed oil possesses a potential photo-protective ability. Nanoemulsions proved to be a promising carrier for the topical delivery of the oil.

Keywords: Anti-oxidant, biochemical studies, central composite design, nanoemulsion loaded gel, nanoemulsions, photoprotection, pomegranate seed oil, ultrasonication.

Jurenka J. Therapeutic applications of pomegranate (Punica granatum L.): A review. Altern Med Rev 2008; 13(2): 128-44.
Sharma J, Maity A. Pomegranate phytochemicals: Neutraceutical and therapeutic values. Fruit Veg Cereal Sci Biotech 2010; 4(2): 56-76.
Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit extracts: A review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol 2012; 143(2): 397-405.
Bhandari PR. Pomegranate (Punica granatum L.). Ancient seeds for modern cure? Review of potential therapeutic applications. Int J Nutr Pharmacol Neurol Dis 2012; 2(3): 171-84.
Li Y, Qi Y, Huang TH, Yamahara J, Roufogalis BD. Pomegranate flower: A unique traditional antidiabetic medicine with dual PPAR-alpha/-gamma activator properties. Diabetes Obes Metab 2008; 10(1): 10-7.
Middha SK, Usha T, Pande V. A review on antihyperglycemic and antihepatoprotective activity of eco-friendly Punica granatum peel waste. J Evid Based Complementary Altern Med 2013; 2013: 1-10.
Fernandesa L, Pereira JA, Lopez-Cort I, Salazarb DM, Ramalhosaa E, Casalc S. Lipid composition of seed oils of different pomegranate (Punica granatum L.) cultivars from Spain. Int J Food Studies 2015; 4: 95-103.
Pereira de Melo IL, Teixeira de Carvalho EB, Mancini-Filho J. Pomegranate seed oil (Punica granatum L.): A source of punicic acid (Conjugated α-Linolenic Acid). J Hum Nutr Food Sci 2014; 2: 1024.
Boroushaki MT, Mollazadeh H, Afshari AR. Pomegranate seed oil: A comprehensive review on its therapeutic effects. Int J Pharm Sci Res 2016; 7(2): 430-42.
Aslam MN, Lansky EP, Varani J. Pomegranate as a cosmeceutical source: Pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol 2006; 103(3): 311-8.
Elagbar ZA, Naik RR, Shakya AK, Bardaweel SK. Fatty acids analysis, antioxidant and biological activity of fixed oil of Annona muricata L. seeds. J Chem 2016; 2016: 1-6.
Houshia OJ, Qutit A. Determination of total polyphenolic antioxidants contents in west-bank olive oil. J Nat Sci 2014; 4: 71-6.
Sangeetha G, Vidhya R. In vitro anti-inflammatory activity of different parts of Pedalium murex (L.). Int J Herb Med 2016; 4: 31-6.
Mathen C, Thergaonkar R, Teredesai M, Soman G, Peter S. Evaluation of anti-elastase and antioxidant activity in antiaging formulations containing Terminalia extracts. Int J Herb Med 2014; 2: 95-9.
Widowati W, Fauziah N, Herdiman H, et al. Antioxidant and antiaging assays of Oryza Sativa extracts, vanillin and coumaric acid. J Nat Rem 2016; 16: 88-99.
Ndlovu G, Fouche G, Tselanyane M, Cordier W, Steenkamp V. In -vitro determination of the anti-aging potential of four southern African medicinal plants. Complement Altern Med 2013; 13: 304.
Mizrahi M, Friedman-Levi Y, Larush L, Frid K, Binyamin O, Dori D, et al. Pomegranate seed oil nanoemulsions for the prevention and treatment of neurodegenerative diseases: the case of genetic CJD. Nanomedicine 2014; 10: 1353-63.
Kotta S, Khan AW, Ansari SH, Sharma RK, Ali J. Formulation of nanoemulsion: A comparison between phase inversion composition method and high-pressure homogenization method. Drug Deliv 2015; 22(4): 455-66.
Das S, Haldar PK, Pramanik G. Formulation and evaluation of herbal gel containing Clerodendron infortunatum leaves extract. Int J Pharm Tech Res 2011; 3: 140-3.
Basha BN, Prakasam K, Goli D. Formulation and evaluation of gel containing fluconazole-antifungal agent. Int J Drug Dev Res 2011; 3: 109-28.
Kumar DS, Sairam R, Anandbabu S, Karpagavalli L, Maheswaran A, Narayanan N. Formulation and evaluation of transdermal patches of salbutamol. Res J Pharm Biol Chem Sci 2012; 3: 1132-9.
Ichihashi M, Ando H, Yoshida M, Niki Y, Matsui M. Photoaging of the skin. J Anti Aging Med 2009; 6(6): 46-59.
Bosset S, Bonnet-Duquennoy M, Barré P, et al. Photoageing shows histological features of chronic skin inflammation without clinical and molecular abnormalities. Br J Dermatol 2003; 149(4): 826-35.
Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ, Fisher GJ. Matrix- degrading metalloproteinases in photoaging. J Investig Dermatol Symp Proc 2009; 14(1): 20-4.
Knott A, Reuschlein K, Lucius R, Stäb F, Wenck H, Gallinat S. Deregulation of versican and elastin binding protein in solar elastosis. Biogerontology 2009; 10(2): 181-90.
Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. Dermatoendocrinol 2012; 4(3): 253-8.
Singh RP, Chidambara Murthy KN, Jayaprakasha GK. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem 2002; 50(1): 81-6.
Kumar S, Bajwa BS, Kuldeep S, Kalia AN. Anti inflammatory activity of herbal plants: A review. Int J Adv Pharm Biol Chem 2013; 2(2): 272-81.
Baccarin T, Mitjans M, Ramos D, Lemos-Senna E, Vinardell MP. Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line. J Photochem Photobiol B 2015; 153: 127-36.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2019
Page: [87 - 102]
Pages: 16
DOI: 10.2174/1573401314666180223134235
Price: $58

Article Metrics

PDF: 9