Objectives: Erythroxylum cuneatum is a plant that belongs to the family of Erythroxylaceae.
It is locally known as “Chinta mula” and found around Southeast Asia. This study identified
the active phytochemicals and antioxidant properties in various extracts derived from dried leaves of
Methods: The tests of phytochemical screening included extracts of ethanol, acetone, hexane and
aqueous. The antioxidant activity was determined by measuring total phenolic content, 2,2-
diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and hydrogen peroxide scavenging
Results: The phytochemical screening of Erythroxylum cuneatum dried leaves revealed the presence
of phenolic compounds namely flavonoids, tannins and total phenols. Alkaloids and saponins were
also detected. The antioxidant activity of the examined extracts varies depending on the solvent
used. Generally, acetone extract showed highest total phenolic content with a value of 2228 µg
GAE/g and highest DPPH radical scavenging activity with IC50 of 1020.00 µg/ml compared to the
standard ascorbic acid of 304.44 µg/ml. Ethanol extract exhibited high hydrogen peroxide activity
with IC50 of 83.09 µg/ml.
Conclusion: This study showed that acetone extract contains highest phenolic compounds and both
ethanol and acetone extracts are a potential source of natural antioxidants.