Background: In the present study, the potential of methanolic leaf extract of Taraxacum officinale
plant as a function of bio-inspired green synthesis for the fabrication of silver nanoparticles
(AgNPs) has been explored.
Methods: The bio-reduction of aqueous silver nitrate (AgNO3) solution was confirmed by visually detecting
the color change from pale yellow to blackish-brown. Maximum absorbance was observed at
420 nm due to the presence of characteristic surface Plasmon resonance of nano silver by UV-visible
spectroscopy. The role of various functional groups in the bio-reduction of silver and chemical transformation
was verified by Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy
(SEM) and energy dispersive X-ray spectroscopy (EDX) predict the shape (rocky, flack type,
ellipsoidal, etc.), size (68 nm) and elemental composition (Ag as a major constituent) of the
biosynthesized AgNPs, respectively.
Results: Transmission electron microscopy (TEM) analysis further corroborated the morphology of the
AgNPs. Color mapping and atomic force microscopy (AFM) confirmed the nano-sized topography.
The dynamic light scattering (DLS) showed the charge, stability, and size of the AgNPs. The generated
AgNPs presented potential antibacterial activities against Gram-positive and Gram-negative bacterial
strains including Staphylococcus aureus, Escherichia coli, and Haemophilus influenzae. The biosynthesized
AgNPs also showed antiproliferative activity against MCF-7 breast cancer cell line in a dosedependent
Conclusion: In conclusion, results clearly indicate that biosynthesized AgNPs could be used as effective
nano drug for treating infectious diseases caused by multidrug resistant bacterial strains in the near future.