Background: The role of intracellular proteins in the pathogenesis of absence epilepsy
were mentioned. These proteins are thought to be related to energy generation, signal transduction,
inflammation processes and membrane conductance.
Objectives: The investigation of protein profile of the genetically epileptic rat brains was the main
subject of this study.
Methods: For this, a 2D-gel electrophoresis based comparative proteome analysis was performed
using thalamus tissue of genetic absence epileptic WAG/Rij and age matched Wistar rats. Regulated
spots displaying differences in their abundance were identified using MALDI-TOF/TOF.
Among the six spots (DHRS9, BR44, HINT1, CREM, SPRE and PDIA3/ERp57) the highest
mascot score was attributed to ERp57 a neuroprotective/neurodegenerative system associated protein.
Western Blot analyses were performed to validate changes occurring at ERp57 in thalamus
and also identify changes in fronto-parietal cortex.
Results: Reductions in the expression levels of ERp57 were detected in the thalamic and the
fronto-parietal brain regions of the WAG/Rij rats in comparison to Wistar rats.
Conclusion: Such difference might be associated with the pathogenic mechanisms dictating the
absence epilepsy. Lower levels of ERp57 may be playing an important role in the development of
spontaneous seizures activity seen in the absence epileptic WAG/Rij rats strain.