Background: The impact of fungal infections on human health has increased considerably
within a past few decades. Although drugs with antifungal properties are available, but they are less effective
and are associated with side effects.
Objective and Method: To screen the bacterial isolates from Sesamum indicum and to investigate the
antifungal activity of the screened bacterial isolates against Aspergillus sp. Co-culture assay and agar
overlay were used to scrutinize the anti-Aspergillus activity. Furthermore, optimization of media and
growth conditions to enhance the production of anti-Aspergillus compound.
Results: Several bacterial cultures were isolated from Sesamum indicum rhizosphere collected from
Mandi (H.P.) India. These bacterial cultures were assayed for antifungal activity against Aspergillus species
i.e. A. fumigatus and A. niger. Two most potent strains were chosen for more detailed analyses. The
biochemical characterization and 16S ribosomal RNA sequencing revealed that Burkholderia sp. strain
RC1 and Acinetobacter pittii strain RC2 exhibit strong similarity (100%) with Burkholderia sp. SR2-07
and Acinetobacter sp. strain 3-59. Additionally, it was also validated that RC1 and RC2 showed
significant difference in the production of anti-Aspergillusactivity under altered growth conditions.
Conclusion: Results from this study recommend that plant rhizosphere remains a rich hotspot for delivering
a novel antifungal compounds.