Background: In recent years, very few effective drugs against Mycobacterium
tuberculosis have emerged, which motivates the research with drugs already
used in the treatment of tuberculosis. Ethambutol is a bacteriostatic drug that affects
cell wall integrity, but the effects of this drug on bacilli are not fully exploited.
Objective: Based on the need to better investigate the complex mechanism of action
of ethambutol, our study presented the proteome profile of M. tuberculosis after different
times of ethambutol exposure, aiming to comprehend the dynamics of bacilli
response to its effects. M. tuberculosis was exposed to ½ MIC of ethambutol at 24
and 48 hours. The proteins were identified by MALDI-TOF/TOF.
Results: The main protein changes occurred in metabolic proteins as dihydrolipoyl
dehydrogenase (Rv0462), glutamine synthetase1 (Rv2220), electron transfer flavoprotein
subunit beta (Rv3029c) and adenosylhomocysteinase (Rv3248c).
Conclusion: Considering the functions of these proteins, our results support that the
intermediary metabolism and respiration were affected by ethambutol and this disturbance
provided proteins that could be explored as additional targets for this drug.