Combined Effects of Salt-related Variables on Qualitative Characteristics of Probiotic Fermented Milk

Author(s): Masoumeh Arab, Sara Sohrabvandi, Nasim Khorshidian, Amir M. Mortazavian*.

Journal Name: Current Nutrition & Food Science

Volume 15 , Issue 3 , 2019

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Graphical Abstract:


Background: Doogh is a typical Iranian beverage based on fermented milk. The common salt used for Doogh is NaCl. Addition of salt in Doogh can take place before or after fermentation. The type of salt (NaCl or NaCl/KCl) and the sequence of salt addition in Doogh production can have significant impacts on biochemical characteristics of this product.

Methods: Nine probiotic Doogh treatments containing two probiotics (Lactobacillus acidophilus and Bifidobacterium lactis BB at the level of 107 cfu.mL-1) along with Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus, different salt percentages (0.5 and/or 1% (w/w)) in two ratios of NaCl/KCl (100:0 or 50:50) were prepared. Four samples were produced with the addition of salt before fermentation and four samples after fermentation (after cooling to 5°C). Apparent viscosity, phase separation, viability of probiotics and sensory properties of Doogh samples were studied during 21 days storage.

Results: The results showed that samples containing 1% (w/w) salt (NaCl or NaCl:KCl) had higher phase separation than treatments with 0.5% (w/w). The partial substitution of NaCl with KCl had a positive influence on the viability of probiotics. Treatments with 1% (w/w) NaCl:KCl and 0.5% (w/w) NaCl:KCl added before fermentation showed the maximum viability of probiotics. Addition of salt decreased the apparent viscosity of Doogh due to decreasing mean particle size of the dispersion system. From sensory point of view, the treatment with 0.5% (w/w) NaCl and those with 0.5% (w/w) NaCl:KCl before fermentation showed the highest acceptance.

Conclusion: A low-sodium Doogh with an acceptable probiotic viability was produced.

Keywords: Doogh, potassium chloride, probiotic, sensory, viability, viscosity.

Tamime AY. Fermented milks: A historical food with modern applications- a review. Eur J Clin Nutr 2002; 56: 2-15.
Yousefi M, Khorshidian N, Mortazavian AM, Hosseini H. A review on the impact of herbal extracts and essential oils on viability of probiotics in fermented milks. Curr Nutr Food Sci 2017; 13: 6-15.
Sanchez B, de los Reyes-Gavilan C, Margolles A, Gueimonde M. Probiotic fermented milks: Present and future. Int J Dairy Technol 2009; 62: 472-83.
Kristo E, Biliaderis CG, Tzanetakis N. Modelling of rheological, microbiological and acidification properties of a fermented milk product containing a probiotic strain of Lactobacillus paracasei. Int Dairy J 2003; 13: 517-28.
Foroughinia S, Abbasi S, Hamidi Z. Effect of individual and combined addition of salep, tragacanth and guar gums on the stabilization of Iranian Doogh. Iran J Nutr Sci Food Technol 2007; 2: 15-25.
Azarikia F, Abbasi S. On the stabilization mechanism of Doogh (Iranian yoghurt drink) by gum tragacanth. Food Hydrocoll 2010; 24: 358-63.
Anon . 2013.Doogh - Specifications and test methods; No. 2453.Available on (In Persian).
Ahmadi E, Mortazavian AM, Fazeli MR, Ezzatpanah H, Mohammadi R. The effects of inoculant variables on the physicochemical and organoleptic properties of Doogh. Int J Dairy Technol 2012; 65: 274-81.
Mohammadi R, Sohrabvandi S, Mortazavian AM. The starter culture characteristics of probiotic microorganisms in fermented milks. Eng Life Sci 2012; 12: 399-409.
Mortazavian AM, Ehsani MR, Azizi A, Razavi SH, Mousavi SM, Sohrabvandi S. Viability of calcium alginate-microencapsulated probiotic bacteria in Iranian yogurt drink (Doogh) during the refrigerated storage period and under the simulated gastrointestinal conditions. Aust J Dairy Technol 2008; 63: 24-9.
Mortazavian AM, Ghorbanipour S, Mohammadifar MA, Mohamadi M. Biochemical properties and viable probiotic population of yogurt at different bacterial inoculation rates and incubation temperatures. Philipp Agric Sci 2011; 94: 111-6.
Mortazavian AM, Khosrokhavar R, Rastgar H, Mortazaei GR. Effects of dry matter standardization order on biochemical and microbiological characteristics of Doogh (Iranian fermented milk drink). Ital J Food Sci 2010; 22: 98-104.
Mortazavian AM, Ehsani MR, Mousavi SM, Rezaei K, Sohrabvandi S, Reinheimer JA. Effect of refrigerated storage temperature on the viability of probiotic micro-organisms in yogurt. Int J Dairy Technol 2007; 60: 123-7.
Mortazavian AM, Ehsani MR, Mousavi SM, et al. Preliminary investigation of the combined effect of heat treatment and incubation temperature on the viability of the probiotic micro-organismsin freshly made yogurt. Int J Dairy Technol 2006; 59: 8-11.
Taylor T, Lathrop A. Evaluation of antimicrobials and salt replacers for use in low-sodium dairy products. J Food Saf 2015; 35: 32-40.
Karagozlu C, Kinik O, Akbulut N. Effects of fully and partial substitution of NaCl by KCl on physico-chemical and sensory properties of white pickled cheese. Int J Food Sci Nutr 2008; 59: 181-91.
Karimi R, Mortazavian AM, Karami M. Incorporation of Lactobacillus casei in Iranian UF-Feta cheese made by partial replacement of NaCl with KCl. J Dairy Sci 2012; 95: 4209-22.
Ayyash MM, Sherkat F, Francis P, Williams RPW, Shah NP. The effect of sodium chloride substitution with potassium chloride on texture profile and microstructure of Halloumi cheese. J Dairy Sci 2011; 94: 37-42.
Sohrabvandi S, Mortazavian AM, Dolatkhah-nejad MR, Bahadori Monfared A. Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yogurt bacteria. Iranian J Biotechnol 2012; 10: 16-21.
Heydari S, Mortazavian AM, Ehsani MR, Mohammadifar MA, Ezzatpanah H, Sohrabvandi S. Biochemical, microbiological and sensory characteristics of probiotic yogurt containing various prebiotic or fiber compounds. Ital J Food Sci 2011; 23: 153-63.
Shafiee G, Mortazavian AM, Mohammadifar MA, Koushki MR, Mohammadi AR, Mohammadi R. Combined effects of dry matter content, incubation temperature and final pH of fermentation on biochemical and microbiological characteristics of probiotic fermented milk. Afr J Microbiol Res 2010; 4: 1265-74.
Khanniri E, Sohrabvandi S, Arab M, Shadnoush M, Mortazavian AM. Effects of stabilizer mixture on physical stability of non-fat Doogh, an Iranian traditional drink. Koomesh 2017; 19: 144-53.
Belitz H, Grosch W, Schieberle P. Food chemistry. 2009. Springer: Berlin Heidelberg
Evans EW. Interactions of Milk Components in Food Systems. In:Birch GG, Lindley MG, EdsInteractions of Food Components. Elsevier: New York. 1985; pp. 245-50.

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Article Details

Year: 2019
Page: [234 - 242]
Pages: 9
DOI: 10.2174/1573401314666180123151007
Price: $65

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