Chemoselective ligation reactions provide facile access to various peptides and
proteins including cyclic peptides, posttranslationally modified proteins, and peptide/
protein probes. Recently, peptide-ligase-based chemo-enzymatic methods have
emerged as promising alternatives to chemical approaches. Ligation mediated by the
newly discovered butelase 1 represents one of the state-of-the-art chemo-enzymatic strategies
for its high catalytic efficiency and unique substrate specificity. The butelasemediated
ligation can be carried out in a semisynthetic manner, in which chemically synthesized
peptides and expressed proteins are used as substrates, rendering it suitable for
various applications including site-specific modification of proteins and the preparation of
thioesters. In this review, we summarize the recent studies and applications of butelase 1.
Keywords: Chemoselective ligation reaction, peptide ligase, butelase 1, peptide/protein drugs, cyclic peptides, protein modification.
Rights & PermissionsPrintExport