Pharmacognostical, Anti-oxidant Activity and High Performance Thin Layer Chromatography Studies on Leaves of Quisqualis indica Linn

Author(s): Mayank Kulshreshtha*, Gunja Srivastava, Manjul Pratap Singh.

Journal Name: Current Traditional Medicine

Volume 4 , Issue 1 , 2018

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Abstract:

Background: Quisqualis indica Linn (Q.indica), family- Combretecea, vining and evergreen plant, is broadly grown in India as ornamental plant in most of the gardens. Traditionally, different parts of this plant (root, seed, fruit) have different uses such as killing of parasitic worms; fruits are also used for nephritis; leaves as to relieve pain; whereas roots are used to treat rheumatism. Pharmacologically, Q. indica have anti-inflammatory, immunomodulatory, anti-staphylococcal, acetylcholinesterase inhibitory activity etc.

Objective: In the present study, micro and macroscopic characteristics of fresh and dried leaves were determined and physiochemical parameters, quantitave microscopy and phytochemical screening were done as per the WHO guidelines. Anti-oxidant activity was done using DPPH method. HPTLC detection was conducted using lupeol and urosolic acid. Standards were scanned at 550 and 522 nm, respectively.

Result: Microscopic parameters (Q. indica) showed the presence of trichomes (different types), stomata calcium oxalate crystals, parencymatous cells, epidermis, phloem and xylem. Total ash value, water soluble ash, acid soluble ash, water soluble extract, alcohol soluble extract, loss on drying, swelling index and foaming index were found to be 7.84±0.79, 3.4±0.02, 1.2±0.01, 60.1±1.75, 19.9±1.30, 7.8±0.79, 2.4±0.33, less than 100. Different quantitative parameters were established. Phytochemical analysis of different extracts showed the presence of different primary and secondary metabolites like alkaloids, glycosides, tannins, saponins, phenolic compounds etc. The IC50 value was found to be 14 and 12.5 in case of ethanolic extract and aqueous extract of Q.indica. HPTLC data showed the presence of both the standards. The amount of Lupeol was found to be 0.011% while Urosolic acid was 0.018%. An attempt was made to explore the proteinaceous nature of selected extracts and was subjected to western blotting. Promising results were obtained as the extract exhibited 70, 50, 55, 48, 35, 30, 25 and 10 kda in all the cases. The obtained protein was found to exhibit impressive antimicrobial activity. Further studies are under progress on selected plants.

Conclusion: The research data generated may serve as a supporting reference for future research work. Researchers may also use a supportive data while preparing monograph. Standardization parameters may be of use in quality control studies on selected plants in the near future.

Keywords: Anti-oxidant, HPTLC, lupeol, Quisqualis indica, ursolic acid, chromatography.

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Article Details

VOLUME: 4
ISSUE: 1
Year: 2018
Page: [53 - 67]
Pages: 15
DOI: 10.2174/2215083804666180118095645

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