Background: Quisqualis indica Linn (Q.indica), family- Combretecea, vining and
evergreen plant, is broadly grown in India as ornamental plant in most of the gardens. Traditionally,
different parts of this plant (root, seed, fruit) have different uses such as killing of
parasitic worms; fruits are also used for nephritis; leaves as to relieve pain; whereas roots are
used to treat rheumatism. Pharmacologically, Q. indica have anti-inflammatory, immunomodulatory,
anti-staphylococcal, acetylcholinesterase inhibitory activity etc.
Objective: In the present study, micro and macroscopic characteristics of fresh and dried
leaves were determined and physiochemical parameters, quantitave microscopy and phytochemical
screening were done as per the WHO guidelines. Anti-oxidant activity was done
using DPPH method. HPTLC detection was conducted using lupeol and urosolic acid. Standards
were scanned at 550 and 522 nm, respectively.
Result: Microscopic parameters (Q. indica) showed the presence of trichomes (different
types), stomata calcium oxalate crystals, parencymatous cells, epidermis, phloem and xylem.
Total ash value, water soluble ash, acid soluble ash, water soluble extract, alcohol soluble
extract, loss on drying, swelling index and foaming index were found to be 7.84±0.79,
3.4±0.02, 1.2±0.01, 60.1±1.75, 19.9±1.30, 7.8±0.79, 2.4±0.33, less than 100. Different quantitative
parameters were established. Phytochemical analysis of different extracts showed the
presence of different primary and secondary metabolites like alkaloids, glycosides, tannins,
saponins, phenolic compounds etc. The IC50 value was found to be 14 and 12.5 in case of
ethanolic extract and aqueous extract of Q.indica. HPTLC data showed the presence of both
the standards. The amount of Lupeol was found to be 0.011% while Urosolic acid was
0.018%. An attempt was made to explore the proteinaceous nature of selected extracts and
was subjected to western blotting. Promising results were obtained as the extract exhibited
70, 50, 55, 48, 35, 30, 25 and 10 kda in all the cases. The obtained protein was found to exhibit
impressive antimicrobial activity. Further studies are under progress on selected plants.
Conclusion: The research data generated may serve as a supporting reference for future research
work. Researchers may also use a supportive data while preparing monograph. Standardization
parameters may be of use in quality control studies on selected plants in the near