Background: Nano-drug delivery systems as mesoporous silica nanoparticles (MSNs)
with unique properties have the potential to improve drug efficacy. The purpose of the present study
was to design of CLM loaded MSNs-NH2 to improve the physicochemical properties and antibacterial
activity with enhancement delivery to infected tissues.
Methods: A large pore amine functionalized MSN (MSNs-NH2) is described here to facilitate delivery
of clarithromycin (CLM) as an antibacterial drug and enhance the efficacy against Gram
positive and Gram negative bacterial samples. Prepared particles were characterized by Scanning
Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), nitrogen adsorption/
desorption isotherms, Fourier Transform Infrared (FT-IR) spectroscopy and X-ray Diffraction (XRD)
spectroscopy. The antimicrobial activity was evaluated by agar well diffusion and broth dilution
methods. Therefore, the biodistribution of FITC-MSNs was investigated by measure the NIR intensity
fluorescent of fluorescent images from whole animal and dissected organs of NMRI mice.
Results: The results showed that the CLM loaded MSNs-NH2 (CLM/MSNs-NH2) were successfully
prepared having good payload and pH-sensitive drug release kinetics. The antimicrobial investigation
against Staphylococcus aureus and Escherichia coli was showed better performance of antimicrobial
activity of these nanoparticles. In vivo and ex vivo fluorescent imaging investigation on
NMRI mice were shown that FITC-MSNs-NH2 accumulated in the liver and kidney and notably in
Conclusion: The CLM/MSNs-NH2 exhibited higher antimicrobial activity and enhanced the possibility
of microbial infection therapy especially at respiratory infections.