Medium Optimization for Recombinant Soluble Arginine Deiminase Expression in Escherichia coli Using Response Surface Methodology

Author(s): Mahboubeh Zarei, Navid Nezafat, Mohammad Hossein Morowvat, Ali Dehshahri, Mohammad Bagher Ghoshoon, Aydin Berenjian, Younes Ghasemi*.

Journal Name: Current Pharmaceutical Biotechnology

Volume 18 , Issue 11 , 2017

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Abstract:

Objective: Optimization of the medium for recombinant arginine deiminase production in E. coli was performed using response surface methodology. This is the first study of optimization of recombinant arginine deiminase production in E. coli by the use of response surface methodology.

Methods: A Mycoplasm arginine deiminase gene was computationally optimized and inserted into pET-3a (+) expression vector. The synthetic pET3a-arginine deiminase construct was cloned and overexpressed in E. coli. The effect of glucose, NH4Cl and MgSO4.7H2O concentrations on the expression of intracellular soluble arginine deiminase was investigated using central composite design (CCD).

Results: The maximum arginine deiminase activity (U/mL) was obtained in optimal concentrations g/L of glucose, 6.6; NH4Cl, 1.81; MgSO4.7H2O, 0.94; KH2PO4, 3.0; Na2HPO4, 6.78; NaCl, 0.5; CaCl2, 0.1 mL/L (1M), which was about 6.6 fold higher than that in M9 standard medium.

Conclusion: The obtained result can be utilized for large-scale production of this enzyme and related recombinant protein.

Keywords: Arginine deiminase, response surface methodology, media optimization, recombinant production, E. coli, Mycoplasma hominis.

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Article Details

VOLUME: 18
ISSUE: 11
Year: 2017
Page: [935 - 941]
Pages: 7
DOI: 10.2174/1389201019666180115144752
Price: $65

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