Background: Molecularly imprinted polymers (MIPs) are synthetic polymers that have a
selective site for a given analyte, or a group of structurally related compounds, that make them ideal
polymers to be used in separation processes.
Objective: An optimized molecularly imprinted polymer was selected and applied for selective extraction
and analysis of clozapine in rat brain tissue.
Methods: A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration
and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and
analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE
process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification
(LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for
clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision
values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion
infusion in reducing the brain concentration of drug was also evaluated.
Results: The data indicated that calibrated method was successfully applied for the analysis of clozapine
in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of
lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration
of drug was determined.
Conclusion: The proposed MISPE method could be applied in the extraction and preconcentration before
HPLC-UV analysis of clozapine in rat brain tissue.