UHPLC-UV Analysis of Morin and Structurally Related Flavonoids with Potential Anticancer Activity

Author(s): Naser F. Al-Tannak*, Nada A. Al-Hasawi, Ladislav Novotny.

Journal Name: Current Pharmaceutical Analysis

Volume 15 , Issue 4 , 2019

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Graphical Abstract:


Background: Flavonoids as secondary metabolites of plants fulfill various functions in cell protection. They are of a considerable scientific interest because of their potentially medical use due to their anticancer, chemoprotective, antimicrobial, antiallergic, anti-inflammatory and antiviral activities.

Objective: The study aimed to develop a new UHPLC-UV method for morin and 2 other structurally related flavonoids - naringenin and kaempferol as the structural similarity of huge numbers of flavonoids does not limit their various biological functions and activities.

Methods: Separation of morin and 2 other structurally related flavonoids - naringenin and kaempferol - was achieved by using BEH C18 (1.7 µm, 2.1 x 50 mm) analytical column (Waters® Acquity UPLC) and a mobile phase composed of 0.05%v/v Formic acid in water and acetonitrile in proportion of 77:23 v/v and pumped at a flow rate of 0.4 ml/min. Column temperature was set at 25 ºC and samples were analyzed (3 µl injection volume) at a wavelength of 340 nm. Waters® Xevo G2-S QToF coupled with Waters® Acquity UPLC system with binary Solvent Manager (I-Class) via electrospray ionization (ESI) interface was used to confirm the identity of the peaks in biological samples.

Results: A rapid and simple UHPLC-UV separation of morin, kaempferol and naringenin is documented including methods validation. The developed method was applied to measuring morin, kaempferol and naringenin in human plasma after a solid phase extraction. Additionally, stability of morin in tissue culture medium was verified. The extraction method and UHPLC-UV elution conditions described provide a practical means to analyze morin, kaempferol and naringenin in biological matrices.

Conclusion: The developed method is fast and highly sensitive. Moreover, the flavonoids used were stable in human plasma for more than 10 days.

Keywords: Morin, naringenin, kaempferol, UHPLC-UV, separation, human plasma stability, UHPLC-ESI-QToF.

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Article Details

Year: 2019
Page: [295 - 304]
Pages: 10
DOI: 10.2174/1573412914666171220154224
Price: $58

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